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| Status |
Public on Oct 15, 2008 |
| Title |
Analysis of the MSX1 downstream signaling network in neuroblastoma |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Neuroblastoma is an embryonal tumour of the peripheral sympathetic nervous system (SNS). One of the master regulator genes for peripheral SNS differentiation, the homeobox transcription factor PHOX2B, is mutated in familiar and sporadic neuroblastomas. Here we report that inducible expression of PHOX2B in the neuroblastoma cell line SJNB-8 down-regulates MSX1, a homeobox gene important for embryonic neural crest development. Inducible expression of MSX1 in SJNB-8 caused inhibition of both cell proliferation and colony formation in soft agar. Affymetrix micro- array and Northern blot analysis demonstrated that MSX1 strongly up-regulated the Delta-Notch pathway. These experiments describe for the first time regulation of the Delta-Notch pathway by MSX1, and connect these genes to the PHOX2B oncogene, indicative of a role in neuroblastoma biology.
Keywords: Delta, Notch, MSX1, neuroblastoma, PHOX2B
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| Overall design |
Time course experiments of two independent clones (K2 and K5), with 2 or 4 time points, respectively.
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| Contributor(s) |
Revet I, Huizenga G, Chan AA, Koster J, Volckmann R, Sluis Pv, Øra I, Versteeg R, Geerts D |
| Citation(s) |
18201699 |
| Submission date |
Oct 16, 2007 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Linda Valentijn |
| Organization name |
Academic Medical Centre, University of Amsterdam
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| Department |
Department of Oncogenomics
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| Street address |
P.O. Box 22700
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| City |
Amsterdam |
| ZIP/Postal code |
1100DE |
| Country |
Netherlands |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA103023 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE9339_RAW.tar |
34.4 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
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