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Series GSE93123 Query DataSets for GSE93123
Status Public on Jan 05, 2017
Title Genomic instability during reprogramming by nuclear transfer is DNA replication dependent
Organism Homo sapiens
Experiment type Genome variation profiling by SNP array
SNP genotyping by SNP array
Summary Somatic cells can be reprogrammed to a pluripotent state by nuclear transfer into oocytes, yet developmental arrest often occurs. While incomplete transcriptional reprogramming is known to cause developmental failure, reprogramming also involves concurrent changes in gene expression, cell cycle progression and nuclear structure. Here we study cellular reprogramming events in human and mouse nuclear transfer (NT) embryos prior to embryonic genome activation. We show that genetic instability marked by frequent chromosome segregation errors and DNA damage arise prior to, and independent of, transcriptional activity. These errors occur following transition through DNA replication and are repaired by BRCA1. In the absence of mitotic nuclear remodeling, DNA replication is delayed and errors are exacerbated in subsequent mitosis. These results demonstrate that independent of gene expression, cell type-specific features of cell cycle progression constitute a barrier sufficient to prevent the transition from one cell type to another during reprogramming.
Overall design 16 Samples
Contributor(s) Chia G, Agudo J, Treff NR, Sauer MV, Billing D, Brown B, Baer R, Egli D
Citation(s) 28263958
Submission date Jan 04, 2017
Last update date Jul 30, 2019
Contact name Nathan R Treff
Phone 973-871-1236
Organization name RMA of NJ
Department Molecular Biology
Lab Suite 100
Street address 111 Madison Ave
City Morristown
State/province NJ
ZIP/Postal code 07960
Country USA
Platforms (1)
GPL3718 [Mapping250K_Nsp] Affymetrix Mapping 250K Nsp SNP Array
Samples (16)
GSM2445048 44_polar body
GSM2445049 45_polar body
GSM2445050 46_polar body
BioProject PRJNA360093

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE93123_RAW.tar 426.9 Mb (http)(custom) TAR (of CEL, CNT)
Processed data provided as supplementary file

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