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Series GSE93116 Query DataSets for GSE93116
Status Public on Jan 04, 2017
Title Genome-wide DNA Methylation Analysis in Lung Fibroblasts Co-cultured with Silica-exposed Alveolar Macrophages
Organism Rattus norvegicus
Experiment type Methylation profiling by high throughput sequencing
Summary In the present study, we used MeDIP coupled with next-generation sequencing to profile the whole genome DNA methylation patterns of fibroblast from the co-culture model in vitro. We analyzed the data to identify possible biological pathways for fibroblast differentiating into myofibroblast as well as the interactions of related genes.
Overall design The co-culture was established of AMs and fibroblasts. Seeding fibroblasts into six-well plates and incubated for one or two days until approximately 60% confluence was achieved. AMs were seeding into the inserts and placed the insert into the six-well dish and then exposed to 100µg/ml SiO2 for 0-, 24-, and 48 hours
Contributor(s) Li J, Hao C, Hou J
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Submission date Jan 04, 2017
Last update date May 15, 2019
Contact name Juan Li
Organization name Zhengzhou University
Department college of public health
Street address kexue road
City Zhengzhou city
State/province Henan
ZIP/Postal code 475001
Country China
Platforms (1)
GPL14844 Illumina HiSeq 2000 (Rattus norvegicus)
Samples (4)
GSM2444064 14317A_1_treat_0hrs
GSM2444065 14317A_2_treat_24rs
GSM2444066 14317A_3_treat_48hrs
BioProject PRJNA360081
SRA SRP096010

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE93116_RAW.tar 1.3 Gb (http)(custom) TAR (of WIG)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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