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Series GSE93107 Query DataSets for GSE93107
Status Public on Feb 07, 2018
Title Transcription factor Hoxb5 reprograms B cells into functional T lymphocytes [RNA-Seq]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Natural hematopoietic stem cells are currently the only cell resource to generate entire T lymphopoiesis in vivo. Altenatively, functional and non-tumorigenic T lymphocytes converted from the other cell types remain unsuccessful. Evidence is still lacking about the concept that 'lineage-switching factors' (LSFs) exist in HSC, whose re-activation in progeny cells can accomplish lineage conversions. Here we show that upon ectopic expression of Hoxb5(a HSC-specific transcription factor), the Pro-/Pre-B cells were induced into functional early T cell progenitors(iETPs) in vivo. The reprogramming process initiated in the bone marrow and accomplished in the thymus at ETP stage. Strikingly, the T lymphocytes derived from iETPs resemble their natural counterparts in transcriptome pattern, hierarchical differentiation, tissue distribution and immune functions. Our observations pave the way for achieving lineage regeneration by direct reprogramming autologous somatic cells in vivo. Further, our success in haematopoietic system may build a paradigm for LSF-mediated regeneration of other tissues.
 
Overall design Mus musculus mRNA profiles of 24 cell types, including HSC, MPP, Mature lineage cells (T cells_B cells_Myeloid cells), Pro-_Pre-_B cells, BM-ETPs, BM-iETPs, Thy-ETPs, Thy-iETPs, DN1, DN2, DN3, DN4, DP, CD4-SP,CD8-SP, iDN1, iDN2, iDN3, iDN4, iDP, iCD4-SP and iCD8-SP. 1000 of each cell type was sorted for RANseq analysis refered to published protocol (PMID:20203668).
 
Contributor(s) Dong Y, Yang D, Wang J
Citation(s) 29434353
Submission date Jan 04, 2017
Last update date May 15, 2019
Contact name Yong Dong
E-mail(s) dongyong@cmc.edu.cn
Organization name Chengdu medical college
Department Immunology
Street address Xindu road 783, Xindu district
City chengdu
State/province sichuan
ZIP/Postal code 610500
Country China
 
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (76)
GSM2443906 HSC rep1
GSM2443907 HSC rep2
GSM2443908 HSC rep3
This SubSeries is part of SuperSeries:
GSE105057 Transcription factor Hoxb5 reprograms B cells into functional T lymphocytes
Relations
BioProject PRJNA360066
SRA SRP095998

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE93107_genes_expression_fpkm.csv.gz 3.9 Mb (ftp)(http) CSV
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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