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Series GSE92641 Query DataSets for GSE92641
Status Public on Dec 23, 2017
Title Uhrf1 is indispensable for normal limb growth by regulating chondrocyte differentiation
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Chondrocyte differentiation is regulated at a transcriptional level and by various hormonal stimuli. Since chondrocyte differentiation is important for normal skeletal growth, inadequate amounts of differentiation can lead to pathological conditions such as osteoarthritis. Transcriptional regulation can be tightly orchestrated by epigenetic regulators. Among these, ubiquitin-like with PHD and RING finger domains 1 (Uhrf1) is reported to have diverse epigenetic functions, including regulation of DNA methylation. However, the physiological functions of Uhrf1 in skeletal tissues remain unclear. Here we show that limb mesenchymal cell-specific Uhrf1 conditional knockout mice (Uhrf1ΔLimb/ΔLimb) exhibit shortened long bones that have morphological deformities due to impaired chondrocyte differentiation and proliferation. RNA-seq performed on primary cultured chondrocytes obtained from control and Uhrf1ΔLimb/ΔLimb mice revealed that expression levels of proliferative chondrocyte marker genes were downregulated, whereas hypertrophic chondrocyte marker genes were upregulated. In addition, gene ontology analyses and Gene Set Enrichment Analysis (GSEA) suggested that limb growth retardation due to compromised chondrocyte differentiation might be caused by increased activity of the focal adhesion signaling pathway. These results indicated that Uhrf1 has a crucial role in normal skeletal maturation by coordinating transcriptional regulatory networks during chondrocyte differentiation.
Overall design mRNA profiles of primary chondrocytes obtained from 3-day old Control and Uhrf1 cKO mice were generated by deep sequencing, in triplicate, using Illumina Miseq. Sequence data were mapped on the mouse genome (mm10) using Tophat and analyzed by Cufflinks.
Contributor(s) Imai Y, Yamashita M
Citation(s) 29180567
Submission date Dec 20, 2016
Last update date May 15, 2019
Contact name Yuuki Imai
Phone +81-89-960-5925
Organization name Ehime University
Department Proteo-Science Center
Lab Division of Integrative Pathophysiology
Street address Shitsukawa
City Toon
State/province Ehime
ZIP/Postal code 791-0295
Country Japan
Platforms (1)
GPL16417 Illumina MiSeq (Mus musculus)
Samples (6)
GSM2433904 Control rep1
GSM2433905 Control rep2
GSM2433906 Control rep3
BioProject PRJNA358213
SRA SRP095416

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Supplementary file Size Download File type/resource
GSE92641_Uhrf1_gene_exp.xlsx 3.2 Mb (ftp)(http) XLSX
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