GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE92484 Query DataSets for GSE92484
Status Public on Nov 21, 2018
Title Expression data from ORMDL3 knockdown in the A549 Human Lung Epithelial cell line
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Asthma is characterized by intermittent inflammation of the airways, airflow limitation and wheeze. The ORMDL3 locus on chromosome 17q21 confers the major genetic susceptibility to childhood asthma. Although sphingolipids are important in immune signalling, the mechanisms of action of ORMDL3 on airway inflammation are incompletely understood.
We used Affymetrix Human Gene 1.1 ST microarrays to detail the global effects of siRNA-mediated ORMDL3 knockdown during the time course of IL1B-induced inflammation in the A549 Human Lung Epithelial cell line.
Overall design A total of 38 samples were analyzed (19 control, 19 ORMDL3 knockdown) across 5 time-points (0, 2, 4, 8 and 10 hours following IL1B stimulation), with a minimum of 3 replicates per condition/time point combination. Differential expression was assessed in Limma (3.22.7). P-values were adjusted for multiple testing by the Benjamini and Hochberg method, controlling the expected FDR below 0.05.
Contributor(s) Zhang Y, Willis-Owen SA, Spiegel S, Lloyd CM, Moffatt MF, Cookson WO
Citation(s) 30339462
Submission date Dec 15, 2016
Last update date Nov 16, 2021
Contact name William Cookson
Organization name Imperial College London
Street address Dovehouse Street
City London
ZIP/Postal code SW3 6LY
Country United Kingdom
Platforms (1)
GPL11532 [HuGene-1_1-st] Affymetrix Human Gene 1.1 ST Array [transcript (gene) version]
Samples (38)
GSM2430490 A549, Control, 0 Hours, Rep 1
GSM2430491 A549, Control, 10 Hours, Rep 1
GSM2430492 A549, Control, 2 Hours, Rep 1
BioProject PRJNA357651

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE92484_RAW.tar 183.3 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap