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Series GSE92316 Query DataSets for GSE92316
Status Public on Dec 01, 2017
Title The transcriptome effect of knocking down EZH2 in TamR MCF7L
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Purpose: Increasing evidence suggests that epigenetic reprogramming contributes significantly to the development of endocrine therapy resistance in breast cancer. The goal of this work is to explore how the histone methyltransferase EZH2 interacts with ER signaling and drives the insensitiveness of breast cancer cells to the antagonistic effect of tamoxifen on ER activity. Therefore, we comprehensively analyzed the transcriptional program regulated by EZH2 in tamoxifen-resistant (TamR) MCF-7 cells.
Methods: TamR MCF-7 cells between passage 142-144 upon were used for this assay. For mRNA-Seq, cells are transfected with scrambled control shRNAs (shCtrl) or shRNAs targeting EZH2 (shEZH2). Total RNA were extracted by TRIzol (Invitrogen) and libraries were constructed using Illumina TruSeq RNA Sample Prep Kit v2 (Cat.# RS-122-2001). Hiseq 3000 was used for sequencing.
Overall design Transcriptome profiles of Tamoxifen resistance MCF-7 infected with shCtrl or shEZH2 were sequenced in duplicate using Illumina HiSeq 3000.
Contributor(s) Wu Y, Zhang Z, Xu K
Citation(s) 29212856
Submission date Dec 13, 2016
Last update date May 15, 2019
Contact name Kexin Xu
Organization name UT Health Science Center at San Antonio
Department Molecular Medicine
Street address 7703 Floyd Curl, MC 8257
City San Antonio
State/province Texas
ZIP/Postal code 78229
Country USA
Platforms (1)
GPL21290 Illumina HiSeq 3000 (Homo sapiens)
Samples (4)
GSM2425620 shCtrl rep1
GSM2425621 shEZH2 rep1
GSM2425622 shCtrl rep2
This SubSeries is part of SuperSeries:
GSE103243 Tamoxifen Resistance in Breast Cancer is Regulated by the EZH2-ERa-GREB1 Transcriptional Axis
BioProject PRJNA357207
SRA SRP095013

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SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE92316_RAW.tar 790.0 Kb (http)(custom) TAR (of TXT)
GSE92316_TamR_MCF7_readCounts.txt.gz 2.3 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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