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Status |
Public on Apr 29, 2008 |
Title |
Expression profiles of human glioblastoma frozen tumors and cell lines |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by array
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Summary |
We have developed a nonheuristic genome topography scan (GTS) algorithm to characterize the patterns of genomic alterations in human glioblastoma (GBM), identifying frequent p18INK4C and p16INK4A codeletion. Functional reconstitution of p18INK4C in GBM cells null for both p16INK4A and p18INK4C resulted in impaired cell-cycle progression and tumorigenic potential. Conversely, RNAi-mediated depletion of p18INK4C in p16INK4A-deficient primary astrocytes or established GBM cells enhanced tumorigenicity in vitro and in vivo. Furthermore, acute suppression of p16INK4A in primary astrocytes induced a concomitant increase in p18INK4C. Together, these findings uncover a feedback regulatory circuit in the astrocytic lineage and demonstrate a bona fide tumor suppressor role for p18INK4C in human GBM wherein it functions cooperatively with other INK4 family members to constrain inappropriate proliferation. Keywords: static expression
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Overall design |
Expression profiles of human glioblastoma frozen tumors and cell lines were obtained to study copy number abberation driven expressin alteration. Affymetrix human genome U133plus2 arrays were used to obtain the expression profiles of human glioblastoma tumors and cell lines.
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Contributor(s) |
Wiedemeyer R, Brennan C, Heffernan TP, Xiao Y, Mahoney J, Protopopov A, Zheng H, Bignell G, Furnari F, Cavenee WK, Hahn WC, Ichimura K, Collins VP, Chu GC, Stratton MR, Ligon KL, Futreal PA, Chin L |
Citation(s) |
18394558 |
Submission date |
Sep 26, 2007 |
Last update date |
Mar 25, 2019 |
Contact name |
Yonghong Xiao |
E-mail(s) |
yonghong_xiao@yahoo.com
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Organization name |
Dana-Farber Cancer Institute
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Street address |
450 Brookline Ave., HIM218A
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City |
Boston |
State/province |
MA |
ZIP/Postal code |
02115 |
Country |
USA |
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Platforms (1) |
GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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Samples (30)
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This SubSeries is part of SuperSeries: |
GSE9200 |
Feedback Circuit among INK4 Tumor Suppressors Constrains Human Glioblastoma Development |
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Relations |
BioProject |
PRJNA105239 |