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Series GSE9171 Query DataSets for GSE9171
Status Public on Apr 29, 2008
Title Expression profiles of human glioblastoma frozen tumors and cell lines
Organism Homo sapiens
Experiment type Expression profiling by array
Summary We have developed a nonheuristic genome topography scan (GTS) algorithm to characterize the patterns of genomic alterations in human glioblastoma (GBM), identifying frequent p18INK4C and p16INK4A codeletion. Functional reconstitution of p18INK4C in GBM cells null for both p16INK4A and p18INK4C resulted in impaired cell-cycle progression and tumorigenic potential. Conversely, RNAi-mediated depletion of p18INK4C in p16INK4A-deficient primary astrocytes or established GBM cells enhanced tumorigenicity in vitro and in vivo. Furthermore, acute suppression of p16INK4A in primary astrocytes induced a concomitant increase in p18INK4C. Together, these findings uncover a feedback regulatory circuit in the astrocytic lineage and demonstrate a bona fide tumor suppressor role for p18INK4C in human GBM wherein it functions cooperatively with other INK4 family members to constrain inappropriate proliferation.
Keywords: static expression
 
Overall design Expression profiles of human glioblastoma frozen tumors and cell lines were obtained to study copy number abberation driven expressin alteration.
Affymetrix human genome U133plus2 arrays were used to obtain the expression profiles of human glioblastoma tumors and cell lines.
 
Contributor(s) Wiedemeyer R, Brennan C, Heffernan TP, Xiao Y, Mahoney J, Protopopov A, Zheng H, Bignell G, Furnari F, Cavenee WK, Hahn WC, Ichimura K, Collins VP, Chu GC, Stratton MR, Ligon KL, Futreal PA, Chin L
Citation(s) 18394558
Submission date Sep 26, 2007
Last update date Mar 25, 2019
Contact name Yonghong Xiao
E-mail(s) yonghong_xiao@yahoo.com
Organization name Dana-Farber Cancer Institute
Street address 450 Brookline Ave., HIM218A
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (30)
GSM231695 Glioblastoma tumor GBM_157
GSM231696 Glioblastoma tumor GBM_186
GSM231697 Glioblastoma tumor GBM_193
This SubSeries is part of SuperSeries:
GSE9200 Feedback Circuit among INK4 Tumor Suppressors Constrains Human Glioblastoma Development
Relations
BioProject PRJNA105239

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Supplementary file Size Download File type/resource
GSE9171_RAW.tar 146.8 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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