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Series GSE90949 Query DataSets for GSE90949
Status Public on Jul 20, 2017
Title Genome-wide maps of Bcl11b bound regions in thymocytes
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary T-cell receptor (TCR) signals play a critical role in guiding selected thymocytes to distinct lineages by activating genes for transcription factors, such as ThPOK and Runx3, for the helper- or cytotoxic-lineage, respectively. Here we show that Bcl11b, known as an early T-lineage commitment factor, is essential for proper expression of ThPOK and Runx3. Loss of Bcl11b resulted in premature and random expression of these specification factors, leading to lineage scrambling that was disconnected from TCR restriction by MHC. Early Thpok repression by Bcl11b is independent of its known transcriptional silencer, but requires the C-terminal zinc-finger of Bcl11b. Collectively, our findings shed new light on the role of Bcl11b in priming lineage-specifying genes to integrate TCR signals into a subsequent developmental program that dissects effector lineages.
Overall design Sequencing of Bcl11b ChIP-seq from thymocytes.
Contributor(s) Taniuchi I, Kojo S, Endo TA
Citation(s) 28951542
Submission date Dec 06, 2016
Last update date May 15, 2019
Contact name Takaho A. Endo
Organization name RIKEN
Department IMS
Lab Laboratory for Integrative Genomics
Street address 1-7-22 Suehiro, Tsurumi
City Yokohama
State/province Kanagawa
ZIP/Postal code 230-0045
Country Japan
Platforms (1)
GPL18480 Illumina HiSeq 1500 (Mus musculus)
Samples (2)
GSM2417981 Bcl11b_ThPOKgfp
GSM2417982 Bcl11b_ThPOKgfpTESPE
This SubSeries is part of SuperSeries:
GSE90990 Bcl11b hypomorphic mutant thymocytes
BioProject PRJNA356425
SRA SRP094644

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE90949_RAW.tar 29.3 Mb (http)(custom) TAR (of WIG)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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