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Series GSE90847 Query DataSets for GSE90847
Status Public on Apr 18, 2017
Title Epithelial-Mesenchymal Micro-Niches Govern Stem Cell Lineage Choices (bulk RNA-Seq)
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Adult tissue stem cells (SCs) reside in niches, which through intercellular contacts and signaling, influence SC behavior. Once activated, SCs typically give rise to short-lived transit-amplifying cells (TACs), which then progress to differentiate into their lineages. Here, using single cell RNA-sequencing, we unearth unexpected heterogeneity among SCs and TACs of hair follicles. We trace the roots of this heterogeneity to micro-niches along epithelial-mesenchymal interfaces, where progenitors display molecular signatures reflective of spatially distinct local signals and intercellular interactions. Using lineage-tracing, temporal single cell analyses and chromatin landscaping, we show that SC plasticity becomes restricted in a sequentially and spatially choreographed program, culminating in seven spatially arranged uni-lineage progenitors within TACs of mature follicles. By compartmentalizing SCs into micro-niches, tissues gain precise control over morphogenesis and regeneration: Some progenitors specify lineages immediately; others retain potency, preserving self-renewing features established early while progressively restricting lineages as they experience dynamic changes in microenvironment.
 
Overall design Mouse hair follicle stem cell, outer root sheath (ORS), basal hair bulb cells, suprabasl hair bulb cells in Anagen-VI were FACS-purified for RNA-seq.
 
Contributor(s) Yang H, Fuchs E
Citation(s) 28413068
Submission date Dec 04, 2016
Last update date May 15, 2019
Contact name Hanseul Yang
E-mail(s) hyang01@rockefeller.edu
Organization name Rockefeller University
Lab Fuchs lab
Street address 1230 York Avenue
City New York
State/province NY
ZIP/Postal code 10065
Country USA
 
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (9)
GSM2415052 Ana-VI_HFSC_RNAseq_rep1
GSM2415053 Ana-VI_HFSC_RNAseq_rep2
GSM2415054 Ana-VI_HFSC_RNAseq_rep3
This SubSeries is part of SuperSeries:
GSE90850 Epithelial-Mesenchymal Micro-Niches Govern Stem Cell Lineage Choices
Relations
BioProject PRJNA356169
SRA SRP094525

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE90847_AnaVI_bulk_RNAseq_TPM.txt.gz 830.7 Kb (ftp)(http) TXT
GSE90847_RAW.tar 13.3 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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