|
| Status |
Public on Nov 22, 2018 |
| Title |
Transcriptomic analysis of intestinal epithelial Sox4 knockout |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Sox4 is expressed in intestinal stem cells (ISCs) and early intestinal progenitors. The regulatory role for Sox4 in intestinal homeostasis is currently unknown. Here, we used RNA-seq to determine the role of Sox4 in the intestinal epithelium using a conditional knockout model.
|
| |
|
| Overall design |
Intestinal epithelium was isolated from adult (6wk) male mice. FACS was used to isolate CD326+ (epithelium-only) cells that were either CD44- (villus enriched) or CD44+ (crypt enriched). 50,000 cells were collected per population. Control mice (Sox4floxed/floxed) were compared to conditional knockout mice (Sox4floxed/floxed:villinCre), which drive recombination of the floxed Sox4 allele starting at E10.5. All mice used were littermates, with 2 biological replicates per control and knockout group.
|
| |
|
| Contributor(s) |
Gracz AD, Magness ST |
| Citation(s) |
30055169 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 DK091427 |
Genetic Control of Intestinal Epithelial Stem Cell Maintenance & Differentiation |
UNIVERSITY OF NORTH CAROLINA AT CHAPEL HILL |
SCOTT T MAGNESS |
|
| Submission date |
Dec 02, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Adam D Gracz |
| E-mail(s) |
agracz@emory.edu
|
| Organization name |
Emory University
|
| Department |
Medicine
|
| Street address |
615 Michael St. NE
|
| City |
Atlanta |
| State/province |
GA |
| ZIP/Postal code |
30322 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
|
| Samples (8)
|
|
| Relations |
| BioProject |
PRJNA355972 |
| SRA |
SRP094461 |
Less...
More...