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Series GSE90795 Query DataSets for GSE90795
Status Public on Nov 22, 2018
Title Transcriptomic analysis of intestinal epithelial Sox4 knockout
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Sox4 is expressed in intestinal stem cells (ISCs) and early intestinal progenitors. The regulatory role for Sox4 in intestinal homeostasis is currently unknown. Here, we used RNA-seq to determine the role of Sox4 in the intestinal epithelium using a conditional knockout model.
Overall design Intestinal epithelium was isolated from adult (6wk) male mice. FACS was used to isolate CD326+ (epithelium-only) cells that were either CD44- (villus enriched) or CD44+ (crypt enriched). 50,000 cells were collected per population. Control mice (Sox4floxed/floxed) were compared to conditional knockout mice (Sox4floxed/floxed:villinCre), which drive recombination of the floxed Sox4 allele starting at E10.5. All mice used were littermates, with 2 biological replicates per control and knockout group.
Contributor(s) Gracz AD, Magness ST
Citation(s) 30055169
NIH grant(s)
Grant ID Grant title Affiliation Name
R01 DK091427 Genetic Control of Intestinal Epithelial Stem Cell Maintenance & Differentiation UNIVERSITY OF NORTH CAROLINA AT CHAPEL HILL SCOTT T MAGNESS
Submission date Dec 02, 2016
Last update date May 15, 2019
Contact name Adam D Gracz
Organization name Emory University
Department Medicine
Street address 615 Michael St. NE
City Atlanta
State/province GA
ZIP/Postal code 30322
Country USA
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (8)
GSM2412765 control_CD44minus_replicate 1
GSM2412766 control_CD44minus_replicate 2
GSM2412767 Sox4cKO_CD44minus_replicate 1
BioProject PRJNA355972
SRA SRP094461

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Supplementary file Size Download File type/resource
GSE90795_RAW.tar 770.0 Kb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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