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Series GSE90794 Query DataSets for GSE90794
Status Public on Nov 09, 2017
Title Genome-wide maps of Cbfb variants bound regions in E12.5 lineage negative fetal liver cells and adult thymocytes.
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Multi-potent hematopietic progenitors must acquire thymus-homing capacity to initiate T lymphocyte development. Despite its importance, the transcriptional program underlying this process remains elusive. Cbfβ forms transcription factor complexes with Runx proteins, and here we sho that Cbfβ2, encoded by an RNA splice variant of the Cbfb gene, is essential for differentiation of IL7R+PIRhi thymic-homing progenitors in the mouse fetal liver. Cbfβ2 binds to cell-type specific enhancers and induces expression of the principal thymus-homing receptor CCR9. Like in mouse, an alternative splicing event generates Cbfβ2-specific mRNA whose products controls CCR9 expression and thymus homing in zebrafish. Our findings indicate that functional diversification of Runx transcription factor complexes via alternative splicing emerged early in evolution to support a key step in T cell development.
Overall design Sequencing of Cbfb ChIP-seq from E12.5 lineage negative fetal liver cells and adult thymocytes.
Contributor(s) Taniuchi I, Kojo S, Endo TA
Citation(s) 28494867, 29343500
Submission date Dec 02, 2016
Last update date Jun 28, 2019
Contact name Takaho A. Endo
Organization name RIKEN
Department IMS
Lab Laboratory for Integrative Genomics
Street address 1-7-22 Suehiro, Tsurumi
City Yokohama
State/province Kanagawa
ZIP/Postal code 230-0045
Country Japan
Platforms (1)
GPL18480 Illumina HiSeq 1500 (Mus musculus)
Samples (6)
GSM2412759 CBFB1_Lin_negative
GSM2412760 CBFB2_Lin_negative
GSM2412761 panCBFB_Lin_negative
BioProject PRJNA355973
SRA SRP094460

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE90794_RAW.tar 88.5 Mb (http)(custom) TAR (of WIG)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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