GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE90742 Query DataSets for GSE90742
Status Public on Jan 03, 2018
Title Clonal analysis of lineage fate in unperturbed hematopoiesis
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The classical tenet of hematopoiesis posits well-accepted lineage trees that arise from progressively restricted oligopotent and unipotent progenitor populations. However, because fate in hematopoiesis has mostly been studied in the context of transplantation, it is unclear whether these lineage branches and such proposed oligopotent progenitors exist in an unperturbed hematopoietic system. Here, we utilize endogenous transposon tagging to trace the fate of thousands of progenitors and stem cells over time to re-evaluate these dogmas. Our results describe a novel clonal roadmap where the megakaryocyte lineage arises independently of lymphoid and myeloid/erythroid fates. Our data also demonstrate that true oligopotency is largely restricted to the multipotent progenitor (MPP) compartment. Analysis of thousands of stem cell and progenitor transcriptomes demonstrates that lineage determination starts at the MPP stage and identifies a functional hierarchy within this population that drives hematopoiesis. Finally, our results demonstrate that long-term hematopoietic stem cells behave physiologically as megakaryocyte lineage progenitors. Our data provide evidence for a substantially revised hematopoietic roadmap, and highlights unique properties of MPPs and HSCs in situ.
Overall design Single-cell mRNA sequencing of hematopoietic stem cells and multipotent progenitor subsets from mouse bone marrow. Sample LTHSC represents 940 single cells. Sample MPP2 represents 776 single cells. Sample MPP3 represents 1372 single cells. Sample MPP4 represents 1016 single cells. Sample STHSC represents 837 single cells.
Contributor(s) Rodriguez-Fraticelli AE, Wolock S, Weinreb CS, Jankovic M, Sun J, Klein AM, Camargo F
Citation(s) 29323290, 32025033
Submission date Dec 01, 2016
Last update date Feb 19, 2020
Contact name Fernando Camargo
Organization name Boston Children's Hospital
Department Stem Cell and Regenerative Biology
Street address 300 Longwood Ave
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (5)
GSM2411664 Long-term HSCs
GSM2411665 MPP subset 2
GSM2411666 MPP subset 3
BioProject PRJNA355645
SRA SRP094420

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE90742_RAW.tar 13.7 Mb (http)(custom) TAR (of CSV)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap