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Series GSE90712 Query DataSets for GSE90712
Status Public on Mar 01, 2017
Title RNA sequencing of CDH1+/THY1+ and CDH1-/THY1+ germline stem cells
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary To futher understand the properties of CDH1+ GSCs and CDH1- GSCs, flow cytometry was used to sort the twopopulations after trypsin digestion and they were compared by total RNA sequencing (RNA-seq). The data clearlyshowed CDH1+ and CDH1- cells as having highly distinct profiles. In particular, numerous epithelial genes (e.g.Dsp, Pkp2, Krt19) were highly expressed in the CDH1+ population. In contrast, most genes known to be generalmarkers of SSCs/undifferentiated spermatogonia were downregulated (e.g. GFRA1 and ID4) or unchanged (e.g. ZBTB16and SALL4) in CDH1- GSCs. Additionally, KEGG pathway analysis revealed that the two populations exhibiteddistinctive activity in several signaling pathways including WNT and TGFb signaling. Notably, Tgfbr1, Smad2, Smad3tended to be lower in CDH1+ GSCs while Smad7, an inhibitor of TGFb signaling, was higher.The results showed thatCDH1+ GSCs were more epithelial in nature compared to CDH1- GSCs and supported the notion that CDH1+ GSCs are areable to partly overcome MET barrier because they may be in an advanced stage of MET.
Overall design Examination of RNA levels in CDH1+/THY1+ and CDH1-/THY1+ GSCs.
Contributor(s) Dann CT, An J
Citation(s) 28065642
Submission date Nov 30, 2016
Last update date Sep 11, 2019
Contact name Aaron Buechlein
Organization name Indiana University
Department Center for Genomics and Bioinformatics
Street address 1001 E 3rd St
City Bloomington
State/province IN
ZIP/Postal code 47405
Country USA
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (6)
GSM2411052 CDH1-/THY1+ RP7
GSM2411053 CDH1-/THY1+ RP5
GSM2411054 CDH1-/THY1+ WT1
BioProject PRJNA355568
SRA SRP094121

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Supplementary file Size Download File type/resource
GSE90712_Thy1_positive_E_Cad_positive_vs_Thy1_positive_E_Cad_negative.DESeq2_Results.tsv.gz 800.2 Kb (ftp)(http) TSV
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Raw data are available in SRA
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