|
| Status |
Public on Sep 21, 2017 |
| Title |
Organism-level analysis of vaccination reveals networks of protection across tissues |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
To study the response of single lung and liver memory CD8+ T cells to reactivation, mice vaccinated subcutaneously with MVA (10^7 PFUs/mouse) were subsequently challenged intranasally with: (1) PBS; (2) WR (10^7 PFUs/mouse); or (3) Vaccinia virus-derived B8R20-27 peptide (20 µg/mouse). Sixteen hours after challenge, single-cell suspensions were generated from lung or liver and lymphocytes were enriched using Percoll density centrifugation prior to immunostaining and cell sorting. Single memory CD8+ T cells from lung and liver were profiled using plate-based or droplet-based single-cell RNA-seq.
|
| |
|
| Overall design |
Three datasets were generated from single memory CD8+ T cells isolated from lung or liver using plate-based (2,423 cells) or droplet-based (7,292) single-cell RNA-seq methods, which included: (i) 947 cells from lung of MVA-vaccinated mice challenged with PBS, WR or B8R20-27 peptide (plate-based – experiment 1); (ii) 1,476 cells from lung or liver of MVA-vaccinated mice challenged with PBS or WR (plate-based – experiment 2); and (iii) 7,292 cells from lung of MVA-vaccinated mice challenged with PBS or WR (droplet-based – experiment 3). Libraries from each experiment were sequenced as a single pool on an Illumina NextSeq500 sequencer.
|
| |
|
| Contributor(s) |
Chevrier N, Kadoki M |
| Citation(s) |
28942919 |
| Submission date |
Nov 30, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Nicolas Chevrier |
| E-mail(s) |
nchevrier@uchicago.edu
|
| Organization name |
The University of Chicago
|
| Department |
Pritzker School of Molecular Engineering
|
| Lab |
Nicolas Chevrier
|
| Street address |
5640 South Ellis Avenue
|
| City |
Chicago |
| State/province |
Illinois |
| ZIP/Postal code |
60637 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
|
| Samples (11)
|
|
| Relations |
| BioProject |
PRJNA355404 |
| SRA |
SRP094109 |
Less...
More...