|
| Status |
Public on May 01, 2017 |
| Title |
ChIP-Seq of constitutively expressed Ndt80-Myc in K. lactis |
| Organism |
Kluyveromyces lactis |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
The purpose of this experiment was to identify the genes bound by Ndt80 in K. lactis when constitutively expressed. Ndt80 was tagged with c-myc, the native Ndt80 promoter was replaced with the K. lactis Gal1 promoter and the protein was immunoprecipitated with a c-myc antibody. Cells were grown overnight in SRaffinose until log-phase, then in SRaffinose + 1.7% Galactose for 5 hours. Each experiment was repeated twice and sequenced on an Illumina HiSeq 4000.
|
| |
|
| Overall design |
K.lactis Ndt80-myc vs. untagged control, with two technical replicates for each
|
| |
|
| Contributor(s) |
Nocedal I |
| Citation(s) |
28327289 |
| |
| Submission date |
Nov 29, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Isabel Nocedal |
| Organization name |
UCSF
|
| Street address |
600 16th St.
|
| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94158 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL22716 |
Illumina HiSeq 4000 (Kluyveromyces lactis) |
|
| Samples (4)
|
|
| Relations |
| BioProject |
PRJNA355301 |
| SRA |
SRP094069 |
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