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| Status |
Public on Sep 30, 2017 |
| Title |
The transcriptome comparison between wildtype Hb9-GFP and Satb1-/-Hb9-GFP retinal ganglion cells (RGCs). |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The goal of this experiment was to define genes differentially expressed between P6 Hb9-GFP and Satb1-/-Hb9-GFP cells, which were dissociated and FACS sorted from mice retinae.
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| Overall design |
Mice retinae from P6 Hb9-GFP and Satb1-/-Hb9-GFP were dissected, GFP labeled RGCs were purified by flow cytometry. Five biological replicates, origniating from different litters, were collected for each genotype. RNA was extracted from ~1000 sorted cells and cDNA was synthesized and amplified using Nugen Ovation RNAseq kit. Libraries were prepared using Nugen Ultralow System V2 kit.
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| Contributor(s) |
Peng Y, Sanes JR |
| Citation(s) |
28781169 |
| Submission date |
Nov 29, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Yi-Rong Peng |
| E-mail(s) |
yirongpeng@gmail.com
|
| Organization name |
Harvard University
|
| Department |
Department of Molecular and Cellular Biology and Center for Brain Science
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| Lab |
Josh Sanes
|
| Street address |
52 Oxford Street
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02138 |
| Country |
USA |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (10)
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| This SubSeries is part of SuperSeries: |
| GSE90673 |
Expression profiles of retinal neuronal cells |
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| Relations |
| BioProject |
PRJNA355286 |
| SRA |
SRP094057 |
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