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Series GSE90652 Query DataSets for GSE90652
Status Public on Sep 30, 2017
Title The transcriptome comparison between wildtype Hb9-GFP and Satb1-/-Hb9-GFP retinal ganglion cells (RGCs).
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The goal of this experiment was to define genes differentially expressed between P6 Hb9-GFP and Satb1-/-Hb9-GFP cells, which were dissociated and FACS sorted from mice retinae.
Overall design Mice retinae from P6 Hb9-GFP and Satb1-/-Hb9-GFP were dissected, GFP labeled RGCs were purified by flow cytometry. Five biological replicates, origniating from different litters, were collected for each genotype. RNA was extracted from ~1000 sorted cells and cDNA was synthesized and amplified using Nugen Ovation RNAseq kit. Libraries were prepared using Nugen Ultralow System V2 kit.
Contributor(s) Peng Y, Sanes JR
Citation(s) 28781169
Submission date Nov 29, 2016
Last update date May 15, 2019
Contact name Yi-Rong Peng
Organization name Harvard University
Department Department of Molecular and Cellular Biology and Center for Brain Science
Lab Josh Sanes
Street address 52 Oxford Street
City Cambridge
State/province MA
ZIP/Postal code 02138
Country USA
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (10)
GSM2409939 WT Hb9 Rep1
GSM2409940 WT Hb9 Rep2
GSM2409942 WT Hb9 Rep3
This SubSeries is part of SuperSeries:
GSE90673 Expression profiles of retinal neuronal cells
BioProject PRJNA355286
SRA SRP094057

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE90652_lgTPM-allSamples.txt.gz 914.3 Kb (ftp)(http) TXT
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Processed data are available on Series record

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