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Series GSE90591 Query DataSets for GSE90591
Status Public on Oct 02, 2017
Title Expansion of SFTPC+ cells from human induced pluripotent stem cell-derived CPMhigh progenitor cells by forming alveolar organoids
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Microarray analysis was performed on CPMhigh progenitor cells, P0 SFTPC+ cells, P2 SFTPC+ cells, and P5 SFTPC+ cells derived form human induced pluripotent stem cells (B2-3 SFTPC-GFP reporter hiPSCs), and parental 201B7 hiPSC-derived proximal airway epithelial cells (PAECs) and primary isolated human adult alveolar epithelial (adult AT2) cells, respectively.
Overall design CPMhigh progenitor cells stepwisely induced from hiPSCs were isolated using fluorescence activated cell sorting (FACS) on day 21. Isolated CPMhigh cells were cocultured with human fetal lung fibroblasts forming alveolar organoids (AOs). P0 SFTPC+ cells were isolated from AOs on Day 35. We further expanded SFTPC+ cells by passaging in AOs. Passaged SFTPC+ cells were isolated on Day 63 and Day 105 (P2 and P5, respectively).
Contributor(s) Yamamoto Y, Gotoh S, Ito I
Citation(s) 28967890
Submission date Nov 28, 2016
Last update date Jan 09, 2018
Contact name Shimpei Gotoh
Organization name Kyoto University
Department Center for iPS cell Research and Application
Lab Gotoh Lab
Street address 53 Kawaharacho, Shogoin, Sakyo-ku
City Kyoto
ZIP/Postal code 606-8507
Country Japan
Platforms (1)
GPL17077 Agilent-039494 SurePrint G3 Human GE v2 8x60K Microarray 039381 (Probe Name version)
Samples (18)
GSM2407277 B2-3_CPM-high_rep1
GSM2407278 B2-3_CPM-high_rep2
GSM2407279 B2-3_CPM-high_rep3
BioProject PRJNA355125

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE90591_RAW.tar 55.4 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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