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Series GSE90564 Query DataSets for GSE90564
Status Public on Feb 28, 2017
Title Paclitaxel resistance in triple negative breast cancer involves novel functional genomic aberrations in the ABCB1 gene.
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Five Triple Negative Breast Cancer cell lines were exposed to increasing concentration of Paclitaxel untill they acquired resistance. In order to identify changes in gene expression associated with resistance to PTX, we performed gene expression profiling on parental and resistant cell lines.
Of ~22000 genes surveyed by microarray analysis, 5.0%, 3.7%, 9.0%, 7.3%, and 5.4% of the genes showed changes in expression of 2-fold or greater (p value < 0.05) in BT20, SUM149, MDA-MB-231, MDA-MB-436 and MDA-MB-468 cell lines, respectively.
Overall design Parental and resistant cells growing were seeded at a density of 0.8x106 per well in 6 well-plates and incubated without drug for 24 hours. Cells were harvested and total RNA from parental and resistant cell lines were extracted using the AllPrep DNA/RNA Mini kit. Four independent RNA extraction from different cell passage was performed for all cell lines except for MDA-MB-468. Three independent replicate is presented for MDA-MB-468 P and MDA-MB-468 R. .
Contributor(s) Ahmadzadeh E, Lefebvre F
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Submission date Nov 27, 2016
Last update date Dec 17, 2018
Contact name Elaheh Ahmadzadeh
Phone 5149981730
Organization name McGill University
Street address 3220 Ridgewood, apt 319
City Montreal
State/province Please select
ZIP/Postal code H3V1B9
Country Canada
Platforms (1)
GPL13607 Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Feature Number version)
Samples (38)
GSM2406860 BT20 Parental 1 N=4
GSM2406861 BT20 parental 2 N=4
GSM2406862 BT20 parental 3 N=4
BioProject PRJNA355042

Download family Format
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE90564_RAW.tar 124.8 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table
Processed data provided as supplementary file

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