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Series GSE90538 Query DataSets for GSE90538
Status Public on Aug 06, 2020
Title Foxa and Hnf4alpha targets during conversion from mouse embryonic fibroblasts (MEFs) to hepatocyte-like (iHep) cells and hepatocytes (Hep).
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary We have found three specific combinations of two transcription factors, comprising Hnf4alpha plus Foxa1, Foxa2 or Foxa3, could convert mouse embryonic fibroblasts (MEFs) into cells that closely resemble hepatocytes in vitro. Then we used ChIP-seq to explore the targets of Foxa and Hnf4alpha during conversion event to iHep cells. Besides we performed for hepatocytes.
Overall design Using anti-Foxa antibodies and an anti-Hnf4alpha antibodies we performed ChIP-Seq for iHep_4a1 cells (Foxa1/Hnf4alpha), iHep_4a2 cells (Foxa2/Hnf4alpha), iHep_4a3 cells (Foxa3/Hnf4alpha), 3 cells 48 hours after injection (48hr_4a1, 48hr_4a2, 48hr_4a3) and hepatocytes (Hep).
Contributor(s) Suzuki A, Ohkawa Y, Nagasaki M, Horisawa K, Ueno K
Citation(s) 32755593
Submission date Nov 25, 2016
Last update date Aug 10, 2020
Contact name Atsushi Suzuki
Organization name Medical Institute of Bioregulation, Kyushu University
Department Division of Organogenesis and Regeneration
Street address 3-1-1 Maidashi, Higashi-ku
City Fukuoka
State/province Fukuoka
ZIP/Postal code 812-8582
Country Japan
Platforms (2)
GPL18480 Illumina HiSeq 1500 (Mus musculus)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (24)
GSM2406450 iHep_4a1_1_EGS_Foxa1_R164
GSM2406451 iHep_4a1_1_EGS_input_R164
GSM2406452 iHep_4a1_2_EGS_Foxa1_R170
BioProject PRJNA355153
SRA SRP094005

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Supplementary file Size Download File type/resource
GSE90538_RAW.tar 2.4 Gb (http)(custom) TAR (of BED, BW)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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