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Series GSE90536 Query DataSets for GSE90536
Status Public on Aug 06, 2020
Title Foxa and Hnf4alpha targets during conversion from mouse embryonic fibroblasts (MEFs) to hepatocyte-like (iHep) cells
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary We have found three specific combinations of two transcription factors, comprising Hnf4alpha plus Foxa1, Foxa2 or Foxa3, could convert mouse embryonic fibroblasts (MEFs) into cells that closely resemble hepatocytes in vitro. Then we used ChIP-seq to explore the targets of Foxa and Hnf4alpha during conversion event to iHep cells.
Overall design Using anti-Foxa antibodies and an anti-Hnf4alpha antibody we performed ChIP-Seq for iHep_4a1 cells (Foxa1/Hnf4alpha), iHep_4a2 cells (Foxa2/Hnf4alpha), iHep_4a3 cells (Foxa3/Hnf4alpha), 3 cells 48 hours after injection (48hr_4a1, 48hr_4a2, 48hr_4a3). Also, using an anti-Ty1-tag antibody we performed ChIP-Seq for 48hr_4a3_del2 cells (Foxa3 del2 mutant fused with Ty1-tag/Hnf4alpha).
Contributor(s) Suzuki A, Ohkawa Y, Nagasaki M, Horisawa K, Ueno K
Citation(s) 32755593
Submission date Nov 25, 2016
Last update date Aug 10, 2020
Contact name Atsushi Suzuki
Organization name Medical Institute of Bioregulation, Kyushu University
Department Division of Organogenesis and Regeneration
Street address 3-1-1 Maidashi, Higashi-ku
City Fukuoka
State/province Fukuoka
ZIP/Postal code 812-8582
Country Japan
Platforms (2)
GPL18480 Illumina HiSeq 1500 (Mus musculus)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (21)
GSM2406404 48hr_4a1_1_Foxa1_R222
GSM2406405 48hr_4a1_1_Hnf4a_R222
GSM2406406 48hr_4a1_1_input_R222
BioProject PRJNA355154
SRA SRP094004

Download family Format
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Supplementary file Size Download File type/resource
GSE90536_RAW.tar 1.4 Gb (http)(custom) TAR (of BED, BW)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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