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Series GSE90534 Query DataSets for GSE90534
Status Public on Aug 06, 2020
Title Transcriptome analysis of the induced hepatocyte-like (iHep) cells
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We have found three specific combinations of two transcription factors, comprising Hnf4alpha plus Foxa1, Foxa2 or Foxa3, could convert mouse embryonic fibroblasts (MEFs) into cells that closely resemble hepatocytes in vitro. Then we used RNA-seq to explore the conversion event to the iHep cells.
 
Overall design RNA-Seq for 6 iHep_4a1 cells (Foxa1/Hnf4alpha), 4 iHep_4a2 cells (Foxa2/Hnf4alpha), 6 iHep_4a3 cells (Foxa3/Hnf4alpha), 6 cells 48 hours after transfection (48hr_4a1, 48hr_4a2, 48hr_4a3), 2 iMEFs transduced with Foxa3 del2 mutant/Hnf4alpha, 2 MEFs, and 2 hepatocytes (Hep).
 
Contributor(s) Suzuki A, Ohkawa Y, Nagasaki M, Horisawa K, Ueno K
Citation(s) 32755593
Submission date Nov 25, 2016
Last update date Aug 10, 2020
Contact name Atsushi Suzuki
Organization name Medical Institute of Bioregulation, Kyushu University
Department Division of Organogenesis and Regeneration
Street address 3-1-1 Maidashi, Higashi-ku
City Fukuoka
State/province Fukuoka
ZIP/Postal code 812-8582
Country Japan
 
Platforms (1)
GPL18480 Illumina HiSeq 1500 (Mus musculus)
Samples (28)
GSM2406363 48hr_4a1_1
GSM2406364 48hr_4a1_2
GSM2406365 48hr_4a2_1
Relations
BioProject PRJNA355155
SRA SRP094003

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE90534_RAW.tar 12.0 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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