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Series GSE90533 Query DataSets for GSE90533
Status Public on Jul 26, 2017
Title An HDAC3-PROX1 Corepressor Module Acts on HNF4α to Control Hepatic Triglycerides
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Summary The histone deacetylase HDAC3 is a critical mediator of hepatic lipid metabolism, and liver-specific deletion of HDAC3 leads to fatty liver. To elucidate the underlying mechanism we developed a method of cross-linking followed by mass spectrometry to define a high-confidence HDAC3 interactome in vivo that includes the canonical NCoR/HDAC3 complex as well as Prospero-related homeobox 1 protein (PROX1). HDAC3 and PROX1 co-localize extensively on the mouse liver genome, and are co-recruited by Hepatocyte Nuclear Factor 4α (HNF4α). The HDAC3-PROX1 module controls the expression of a gene program regulating lipid homeostasis, and hepatic-specific ablation of either component increases triglyceride content in liver. These findings underscore the importance of specific combinations of transcription factors and coregulators in the fine tuning of organismal metabolism.
Overall design Biological replicates were uploaded in separated files and indicated in the file names.
Contributor(s) Armour SM, Remsberg JR, Damle M, Sidoli S, Ho WY, Li Z, Garcia BA, Lazar MA
Citation(s) 28916805
Submission date Nov 25, 2016
Last update date May 15, 2019
Contact name Manashree Damle
Organization name Massachusetts General Hospital
Department Molecular Biology
Lab Bob Kingston
Street address 185 Cambridge Street
City Boston
State/province MA
ZIP/Postal code 02155
Country USA
Platforms (2)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (34)
GSM2406329 HDAC3_ChIP-seq_ZT10_Control_rep1
GSM2406330 HDAC3_ChIP-seq_ZT10_Control_rep2
GSM2406331 HDAC3_ChIP-seq_ZT10_Control_rep3
BioProject PRJNA354961
SRA SRP093924

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SOFT formatted family file(s) SOFTHelp
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Supplementary file Size Download File type/resource
GSE90533_Cuffdiff_HDAC3_KO_PROX1_KD.xlsx 3.7 Mb (ftp)(http) XLSX
GSE90533_HDAC3_peaks_1.5rpm.bed.gz 190.9 Kb (ftp)(http) BED
GSE90533_HNF4a_peaks_2rpm.bed.gz 304.7 Kb (ftp)(http) BED
GSE90533_PROX1_peaks_2rpm.bed.gz 246.3 Kb (ftp)(http) BED
GSE90533_RAW.tar 3.8 Gb (http)(custom) TAR (of BIGWIG)
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Raw data are available in SRA
Processed data are available on Series record
Processed data provided as supplementary file

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