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Series GSE90509 Query DataSets for GSE90509
Status Public on Dec 31, 2017
Title Neuronal activity, TGFβ signaling and unpredictable chronic stress affect transcription of Gadd45 family members in vitro and in vivo [MeDIP-Seq]
Organism Mus musculus
Experiment type Methylation profiling by high throughput sequencing
Summary Neuronal activity is altered in several neurological and psychiatric diseases. Upon depolarization not only neurotransmitters are released but also cytokines and other activators of signaling cascades. Unraveling their complex implication in transcriptional control in receiving cells will contribute to understand specific central nervous system (CNS) pathologies and will be of therapeutical interest. In this study we depolarized mature hippocampal neurons in vitro using KCl and revealed increased release not only of Brain-derived neurotrophic factor (BDNF), but also of Transforming growth factor beta (TGFB). Neuronal activity together with BDNF and TGFB controls transcription of DNA modifying enzymes specifically members of the DNA-damage-inducible (Gadd) family, Gadd45a, Gadd45b, and Gadd45g. MeDIP followed by massive parallel sequencing and transcriptome analyses revealed less DNA methylation upon KCl treatment. Thereby, psychiatric disorder-related genes, namely Tshz1, Foxn3, Jarid2, Per1, Map3k5, and Arc are transcriptionally activated and demethylated upon neuronal activation. To analyze whether misexpression of Gadd45 family members can be associated with psychiatric diseases, we applied unpredictable chronic mild stress (UCMS) as established model for depression to mice.UCMS lead to a reduced expression of Gadd45 family members. Taken together, our data demonstrate that Gadd45 family members are new putative targets for UCMS treatments.
Overall design Definition of activity dependent targets on transcriptional and DNA methylation level
Contributor(s) Franz H, Bovio P, Grassi D, Vogel T, Vezzali R
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Submission date Nov 25, 2016
Last update date May 15, 2019
Contact name Henriette Franz
Organization name University Freiburg
Department Anatomy and Cellbiology
Lab Vogel
Street address Alberstr: 17
City Freiburg
ZIP/Postal code 79104
Country Germany
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (7)
GSM2405855 Input_Hippocampal_neurons_pooled
GSM2405856 MeDIP_Hippocampal_neurons_untreated_rep1
GSM2405857 MeDIP_Hippocampal_neurons_untreated_rep2
This SubSeries is part of SuperSeries:
GSE90513 Neuronal activity, TGFβ signaling and unpredictable chronic stress affect transcription of Gadd45 family members in vitro and in vivo
BioProject PRJNA354944
SRA SRP093914

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Supplementary file Size Download File type/resource
GSE90509_RAW.tar 467.0 Mb (http)(custom) TAR (of BED, BW)
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Raw data are available in SRA
Processed data provided as supplementary file

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