|
| Status |
Public on Dec 25, 2007 |
| Title |
Regulation of gene expression by MafA phosphorylation in transformed cells. |
| Organism |
Gallus gallus |
| Experiment type |
Expression profiling by array
|
| Summary |
Transformation of Chicken Embryo fibroblast by MafA is dependent on its phosphorylation status. Using microarray analysis, we identify a gene expression subprogram regulated by MafA phosphorylation.
Keywords: phosphorylation mediated transcriptional regulation
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| |
|
| Overall design |
Total RNA was extracted from Chicken Embryo Fibroblasts expressing control (RCAS), MafA-WT, MafA-4A using RNeasy kit (Qiagen). RNA from two independent cultures for each condition were analyzed and processed by the genomic facility of the Fred Hutchinson Cancer Research Center (Seattle) using a 13K chicken microarray
(http://www.fhcrc.org/science/shared_resources/genomics/dna_array). For one set of sample, dye swap experiments were performed.
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| |
|
| Contributor(s) |
Rocques N, Eychene A, Pouponnot C |
| Citation(s) |
18042454 |
| |
| Submission date |
Sep 14, 2007 |
| Last update date |
Mar 17, 2012 |
| Contact name |
CELIO POUPONNOT |
| E-mail(s) |
celio.pouponnot@curie.u-psud.fr
|
| Phone |
33 1 69863079
|
| Fax |
33 1 69074525
|
| URL |
http://www.curie.fr/equipe/85
|
| Organization name |
Institut Curie
|
| Department |
CNRS UMR 146
|
| Lab |
EYCHENE
|
| Street address |
Bat 110, Centre Universitaire
|
| City |
ORSAY Cedex |
| ZIP/Postal code |
91405 |
| Country |
France |
| |
|
| Platforms (1) |
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| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA102551 |
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