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Series GSE90145 Query DataSets for GSE90145
Status Public on Nov 29, 2016
Title Expression data from triple negative breast cancer cells
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Treatment of triple-negative breast cancer has been challenging and paclitaxel resistance is one of the major obstacles to the better prognosis. Misregulation of alternative splicing (AS) may contribute to tumor progression and chemotherapy resistance. Human AS factor TRA2 has two separate gene paralogs encoding TRA2A and TRA2B proteins. TRA2B is associated with cancer cell survival and therapeutic sensitivity.
We used microarrays to detail the global programme of gene expression and alternative splicing events underlying paclitaxel treatment and TRA2A upregulation and identified distinct classes of up-regulated and down-regulate genes as well as alternative splicing genes during this process.
 
Overall design Breast cancer cell MDA-MB-231 was given paclitaxel treatment and exogenous TRA2A expression before RNA extraction and hybridization on Affymetrix microarrays. Then we get three groups of samples: Control, PTX (MDA-MB-231 with paclitaxel treatment), 231T (MDA-MB-231 with exogenous TRA2A expression).
 
Contributor(s) Liu T, Sun B
Citation(s) 28416606, 29910679
Submission date Nov 22, 2016
Last update date May 21, 2019
Contact name Tieju Liu
E-mail(s) liutieju@tmu.edu.cn
Phone 8633-83336813
Organization name Tianjin Medical University
Department Pathology
Lab Pathology
Street address Qixiangtai Road No.22, Heping District
City Tianjin
State/province Tianjin
ZIP/Postal code 300070
Country China
 
Platforms (2)
GPL17585 [HTA-2_0] Affymetrix Human Transcriptome Array 2.0 [probe set (exon) version]
GPL17586 [HTA-2_0] Affymetrix Human Transcriptome Array 2.0 [transcript (gene) version]
Samples (18)
GSM2399716 control-1 [exon-level]
GSM2399717 control-2 [exon-level]
GSM2399718 control-3 [exon-level]
Relations
BioProject PRJNA355076

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE90145_RAW.tar 506.5 Mb (http)(custom) TAR (of CEL, CHP)
Processed data included within Sample table
Processed data provided as supplementary file

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