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Series GSE90094 Query DataSets for GSE90094
Status Public on Sep 21, 2017
Title The Ets-family transcription factors PU.1 and SpiB are redundantly required by mature B cells to sense and respond to environmental cues
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Humoral immunity requires B cells to respond to multiple stimuli, including antigen, membrane and soluble ligands and microbial products. Ets-family transcription factors regulate many aspects of hematopoiesis, although their roles in humoral immunity have proven difficult to decipher, potentially due to redundancy between the family members. Here we show that mice lacking both PU.1 and SpiB in mature B cells are unable to respond protein antigen, preventing germinal center formation and high-affinity antibody production. Mutant B cells also showed impaired survival following engagement of the CD40 and TLR pathways, but paradoxically enhanced plasma cell differentiation. PU.1 and SpiB control the expression of many components of the BCR signaling pathway and the receptors for CD40L, BAFF and toll ligands. Thus PU.1 and SpiB function enables B cells to appropriately respond to environmental cues.
Overall design Transcriptional profiling of PU.1-/- and SpiB-/- activated B cells using RNA sequencing
Contributor(s) Willis SN, Liao Y, Shi W, Nutt SL
Citation(s) 29127283
Submission date Nov 21, 2016
Last update date Jul 25, 2021
Contact name Wei Shi
Organization name Olivia Newton John Cancer Research Institute
Department Bioinformatics and Cancer Genomics
Street address Level 5, ONJ Cancer Centre, 145 Studley Rd
City Heidelberg
State/province VIC
ZIP/Postal code 3084
Country Australia
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (24)
GSM2397586 Control rep1
GSM2397587 PU.1 cKO rep1
GSM2397588 SpiB KO rep1
BioProject PRJNA354406
SRA SRP093748

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE90094_RAW.tar 5.0 Mb (http)(custom) TAR (of TXT)
GSE90094_Supp_Raw_Counts_trimmed.txt.gz 413.5 Kb (ftp)(http) TXT
GSE90094_supp_raw_counts.txt.gz 655.5 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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