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Series GSE8987 Query DataSets for GSE8987
Status Public on Jul 03, 2008
Title Expression data from buccal and nasal epithelium of current and never smokers
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Smoking is the leading cause of lung cancer death, although only a small percentage of smokers develop the disease. Cigarette smoke exposure is known to cause a field of injury in cells throughout the respiratory tract, and while these airway epithelial cells are morphologically normal, they can undergo genetic alterations in response to cigarette smoke exposure.
We used microarrays to analyze the gene expression of epithelial cells in the extrathoracic epithelium, specifically nasal and buccal epithelium, to see if these cells underwent similar genetic alterations in response to tobacco exposure as seen in bronchial epithelial cells as has been previously reported.
Keywords: cross sectional
Overall design Buccal and nasal epithelial cell samples were collected from healthy current and never smokers. RNA was isolated from these samples and hybridized to Affymetrix microarrays. Gene expression from never smokers was compared to never smoker gene expression from bronchial epithelium as well as expression data from other tissues to determine commonalities in expression patterns in normal extra- and intra-thoracic samples. In addition, gene expression from smokers and nonsmokers was compared in bronchial, nasal, and buccal epithelium to determine similarities in gene expression in these tissues in response to cigarette smoker exposure.
Contributor(s) Spira A, Sridhar S
Citation(s) 18513428
Submission date Sep 07, 2007
Last update date Dec 06, 2018
Contact name Sriram Sridhar
Phone 6178771485
Organization name MedImmune
Department Translational Medicine
Street address 1 MedImmune Way
City Gaithersburg
State/province MD
ZIP/Postal code 20878
Country USA
Platforms (2)
GPL96 [HG-U133A] Affymetrix Human Genome U133A Array
GPL571 [HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array
Samples (25)
GSM227858 Mouth_1 (Never Smoker)
GSM227859 Mouth_2 (Never Smoker)
GSM227860 Mouth_3 (Never Smoker)
BioProject PRJNA102453

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Supplementary file Size Download File type/resource
GSE8987_RAW.tar 220.2 Mb (http)(custom) TAR (of CEL, CHP, EXP)
Processed data included within Sample table
Processed data provided as supplementary file

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