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Series GSE89754 Query DataSets for GSE89754
Status Public on Feb 21, 2018
Title Population snapshots predict early haematopoietic and erythroid hierarchies
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The formation of red blood cells begins with the differentiation of multipotent haematopoietic progenitors. Reconstructing the steps of this differentiation represents a general challenge in stem-cell biology. Here we used single-cell transcriptomics, fate assays and a theory that allows the prediction of cell fates from population snapshots, to demonstrate that mouse hematopoietic progenitors differentiate through a continuous, hierarchical structure into seven blood lineages. We uncovered coupling between the erythroid and the basophil or mast cell fates, a global haematopoietic response to erythroid stress and novel growth factor receptors that regulate erythropoiesis. We defined a flow cytometry sorting strategy to purify early stages of erythroid differentiation, completely isolating classically defined burst-forming and colony-forming progenitors. We also found that the cell cycle is progressively remodelled during erythroid development and during a sharp transcriptional switch that ends the colony-forming progenitor stage and activates terminal differentiation. Our work showcases the utility of linking transcriptomic data to predictive fate models, and provides insights into lineage development in vivo.
Overall design Single-cell mRNA sequencing of hematopoietic progenitors from mouse bone marrow and fetal liver.
Sample Bone marrow, basal: 5432 single cells.
Sample Bone marrow, Epo-stimulated: 2954 single cells.
Sample Fetal liver: 9482 single cells.
Sample P1: 3104 single cells.
Sample P1-CD71hi: 752 single cells.
Sample P2: 2435 single cells.
Sample P3: 2032 single cells.
Sample P4: 1210 single cells.
Sample P5: 2460 single cells.
Contributor(s) Khoramian Tusi B, Wolock SL, Weinreb C, Hwang Y, Hidalgo D, Zilionis R, Waisman A, Huh JR, Klein AM, Socolovsky M
Citation(s) 29466336, 29463712
Submission date Nov 10, 2016
Last update date Aug 26, 2019
Contact name Merav Socolovsky
Organization name University of Massachusetts Medical School
Department Molecular, Cell and Cancer Biology
Street address 364 Plantation St
City Worcester
State/province MA
ZIP/Postal code 01605
Country USA
Platforms (2)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (9)
GSM2388072 Bone marrow, basal
GSM2388073 Bone marrow, Epo-stimulated
GSM2388074 Fetal liver
BioProject PRJNA353098
SRA SRP093236

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Supplementary file Size Download File type/resource
GSE89754_RAW.tar 153.8 Mb (http)(custom) TAR (of CSV)
GSE89754_rawfilenames_with_SRR.txt.gz 709 b (ftp)(http) TXT
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Raw data are available in SRA
Processed data provided as supplementary file

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