|
| Status |
Public on Dec 18, 2019 |
| Title |
Hippo Pathway Regulates Microarchitecture of Fibroblastic Reticular Cells in Lymph Node |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
To unveil the role of Hippo signaling in regulating the maintenance and integrity of FRCs in LNs.
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| |
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| Overall design |
FRCs were sorted from models deleting Yap/Taz to inhibit hippo pathway, and deleting Lats1,2 to activate hippo pathway in FRC specific manner.
We generated [1] FRC-driven Lats1/2 depleted mouse by crossing the PDGFRβ-Cre-ERT2 mouse with Lats1(flox/flox)/Lats2(flox/flox) mouse [2] FRC-driven Yap/Taz depleted mouse by crossing the FRC-specific CCL19-Cre mouse and the double Yap(flox/flox)/Taz (flox/flox) mouse.
Lgr5+ stem cells in dermis were FACS-purified and used for ATAC-seq to explore Yap/Taz driven changes in chromatin accessibility
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| |
|
| Contributor(s) |
Koh GY, Bae H, Yang M, Lee D |
| Citation(s) |
31980640 |
| Submission date |
Nov 10, 2016 |
| Last update date |
Feb 03, 2020 |
| Contact name |
Gou Young Koh |
| Organization name |
IBS-KAIST Center for Vascular Research
|
| Department |
Institue for Basic Science
|
| Street address |
Room 6101, Biomedical Research Center, E7, KAIST 291, Daehak-ro, Yuseong-gu
|
| City |
Daejeon |
| ZIP/Postal code |
34141 |
| Country |
South Korea |
| |
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| Platforms (2) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
|
| Samples (17)
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| Relations |
| BioProject |
PRJNA353070 |
| SRA |
SRP093269 |
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