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Series GSE89742 Query DataSets for GSE89742
Status Public on Dec 18, 2019
Title Hippo Pathway Regulates Microarchitecture of Fibroblastic Reticular Cells in Lymph Node
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Summary To unveil the role of Hippo signaling in regulating the maintenance and integrity of FRCs in LNs.
Overall design FRCs were sorted from models deleting Yap/Taz to inhibit hippo pathway, and deleting Lats1,2 to activate hippo pathway in FRC specific manner.
We generated [1] FRC-driven Lats1/2 depleted mouse by crossing the PDGFRβ-Cre-ERT2 mouse with Lats1(flox/flox)/Lats2(flox/flox) mouse [2] FRC-driven Yap/Taz depleted mouse by crossing the FRC-specific CCL19-Cre mouse and the double Yap(flox/flox)/Taz (flox/flox) mouse.
Lgr5+ stem cells in dermis were FACS-purified and used for ATAC-seq to explore Yap/Taz driven changes in chromatin accessibility
Contributor(s) Koh GY, Bae H, Yang M, Lee D
Citation(s) 31980640
Submission date Nov 10, 2016
Last update date Feb 03, 2020
Contact name Gou Young Koh
Organization name IBS-KAIST Center for Vascular Research
Department Institue for Basic Science
Street address Room 6101, Biomedical Research Center, E7, KAIST 291, Daehak-ro, Yuseong-gu
City Daejeon
ZIP/Postal code 34141
Country South Korea
Platforms (2)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (17)
GSM2387474 LATS1,2 WT1
GSM2387475 LATS1,2 WT2
GSM2387476 LATS1,2 WT3
BioProject PRJNA353070
SRA SRP093269

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE89742_RAW.tar 1.7 Gb (http)(custom) TAR (of BED, TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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