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Series GSE89619 Query DataSets for GSE89619
Status Public on Nov 08, 2016
Title Genome-wide analysis of transcription in in vitro- and in vivo-derived motor neurons
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary RNA sequencing analysis of Hb9::GFP mouse embryonic fibroblasts, Hb9::GFP+ primary mouse embryonic motor neurons at day E13.5, Hb9::GFP+ mouse embryonic stem cell-derived motor neurons, Hb9::GFP+ mouse induced pluripotent stem cell derived motor neurons, and Hb9::GFP+ mouse induced motor neurons generated using transcription factor overexpression. The goal of this project is to evaluate the ability of directed differentiation and lineage conversion techniques to generate a bona fide neuronal subtype.
Overall design The goal of this study is to determine the level of transcriptional similarity between spinal motor neurons generated from pluripotent stem cells by directed differentation, spinal motor neurons generated from fibroblasts by transcription factor-mediated lineage conversion, and primary spinal motor neuron from embryonic spinal cord. In addition, we tested the effect of culturing ESC-MNs in glia-conditioned media. All neurons were generated using the same Hb9::GFP, C57Bl/6 background, and FACS sorted for Hb9::GFP.
Contributor(s) Ichida J, Davis-Dusenberry B
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Submission date Nov 07, 2016
Last update date May 15, 2019
Contact name Justin Ichida
Phone 6172717366
Organization name University of Southern California
Street address 1425 San Pablo St. Bcc 307
City Los Angeles
State/province CA
ZIP/Postal code 90033
Country USA
Platforms (2)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (27)
GSM2385557 GFP+ d13.5 m Embryonic MN 12-6-11
GSM2385558 C57Bl6 Hb9::GFP embryo-MNs E13.5 2, sorted
GSM2385559 C57Bl6 Hb9::GFP mESC-MNs day 7 2, sorted
BioProject PRJNA352722
SRA SRP092781

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Supplementary file Size Download File type/resource
GSE89619_RAW.tar 5.9 Mb (http)(custom) TAR (of TXT)
GSE89619_new6_count_delXY_delLC_4GEO.csv.gz 209.4 Kb (ftp)(http) CSV
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Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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