 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Nov 08, 2016 |
| Title |
Genome-wide analysis of transcription in in vitro- and in vivo-derived motor neurons |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
RNA sequencing analysis of Hb9::GFP mouse embryonic fibroblasts, Hb9::GFP+ primary mouse embryonic motor neurons at day E13.5, Hb9::GFP+ mouse embryonic stem cell-derived motor neurons, Hb9::GFP+ mouse induced pluripotent stem cell derived motor neurons, and Hb9::GFP+ mouse induced motor neurons generated using transcription factor overexpression. The goal of this project is to evaluate the ability of directed differentiation and lineage conversion techniques to generate a bona fide neuronal subtype.
|
| |
|
| Overall design |
The goal of this study is to determine the level of transcriptional similarity between spinal motor neurons generated from pluripotent stem cells by directed differentation, spinal motor neurons generated from fibroblasts by transcription factor-mediated lineage conversion, and primary spinal motor neuron from embryonic spinal cord. In addition, we tested the effect of culturing ESC-MNs in glia-conditioned media. All neurons were generated using the same Hb9::GFP, C57Bl/6 background, and FACS sorted for Hb9::GFP.
|
| |
|
| Contributor(s) |
Ichida J, Davis-Dusenberry B |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Nov 07, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Justin Ichida |
| E-mail(s) |
cheeda1@gmail.com
|
| Phone |
6172717366
|
| Organization name |
University of Southern California
|
| Street address |
1425 San Pablo St. Bcc 307
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90033 |
| Country |
USA |
| |
|
| Platforms (2) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
|
| Samples (27)
|
| GSM2385557 |
GFP+ d13.5 m Embryonic MN 12-6-11 |
| GSM2385558 |
C57Bl6 Hb9::GFP embryo-MNs E13.5 2, sorted |
| GSM2385559 |
C57Bl6 Hb9::GFP mESC-MNs day 7 2, sorted |
| GSM2385560 |
GFP+ mES MN sorted 9-1-11 |
| GSM2385561 |
Hb9::GFP Bl6 mESC rep1 |
| GSM2385562 |
Hb9::GFP Bl6 mESC rep2 |
| GSM2385563 |
Hb9::GFP Bl6 iMN 7F d15 9-24-12 |
| GSM2385564 |
C57Bl6 Hb9::GFP iMNs day 15 2, sorted (not stringent FACS gating) |
| GSM2385565 |
GFP+ 7F iMN day 14 12-20-11 |
| GSM2385566 |
C57Bl6 Hb9::GFP miPSC-MNs day 7 1, sorted |
| GSM2385567 |
C57Bl6 Hb9::GFP miPSC-MNs day 7 2, sorted |
| GSM2385568 |
Hb9::GFP Bl6 iPSC OKS rep1 |
| GSM2385569 |
Hb9::GFP Bl6 iPSC OKS rep2 |
| GSM2385570 |
Hb9::GFP Bl6 MEF FBS medium, not passaged, harvested day 15 1 |
| GSM2385571 |
Hb9::GFP Bl6 MEF FBS medium, not passaged, harvested day 15 2 |
| GSM2385572 |
Hb9::GFP Bl6 MEF N3 neuronal serum-free medium, not passaged, harvested day 15 1 |
| GSM2385573 |
Hb9::GFP Bl6 MEF N3 neuronal serum-free medium, not passaged, harvested day 15 2 |
| GSM2385574 |
Hb9::GFP Bl6 MEF FBS medium, passaged once, harvested day 15 1 |
| GSM2385575 |
Hb9::GFP Bl6 MEF FBS medium, passaged once, harvested day 15 2 |
| GSM2385576 |
C57Bl6 Hb9::GFP MEFs day 2 (FBS) 1 |
| GSM2385577 |
C57Bl6 Hb9::GFP MEFS day 2 (FBS) 2 |
| GSM2912665 |
ESC_MN_CM_rep1 |
| GSM2912666 |
ESC_MN_CM_rep2 |
| GSM2912667 |
ESC_MN_nCM_rep1 |
| GSM2912668 |
ESC_MN_nCM_rep2 |
| GSM2912669 |
E11-5_EMB_MN_rep1 |
| GSM2912670 |
E11-5_EMB_MN_rep2 |
|
| Relations |
| BioProject |
PRJNA352722 |
| SRA |
SRP092781 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE89619_RAW.tar |
5.9 Mb |
(http)(custom) |
TAR (of TXT) |
| GSE89619_new6_count_delXY_delLC_4GEO.csv.gz |
209.4 Kb |
(ftp)(http) |
CSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
| Processed data are available on Series record |
|
|
|
|
 |
Less...
More...