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Status |
Public on Nov 04, 2016 |
Title |
A new model for long-range chromatin reorganization linked to enhancer activation [Agilent_RNA] |
Organism |
Mus musculus |
Experiment type |
Expression profiling by genome tiling array
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Summary |
The most prominent model for long-range enhancer regulation involves direct enhancer-promoter interaction by looping out the intervening chromatin. Using a synthetic biology approach, we have determined that a chromatin unfolding bteween Shh and its enhancers is regulated specifically by the Shh-Brain-Enhancers and is mediated by the recruitment of Poly (ADP-Ribose) Polymerase 1. This ‘chromatin unfolding’ model represents a new mechanism of long-range enhancer-promoter communication in addition to the looping and tracking models. RNA-on-chip for probing nuclear RNA on the Shh regulatory region on ESCs and NPC showed no spreading of nuclear RNA in the regulatory region upon Shh activation.
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Overall design |
Comparison of ESCs and NPC nuclear RNA profiles on Shh region
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Contributor(s) |
Benabdallah NS, Williamson I, Illingworth RS, Boyle S, Grimes GR, Therizols P, Bickmore WA |
Citation missing |
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Submission date |
Nov 03, 2016 |
Last update date |
Jun 13, 2018 |
Contact name |
Nezha Suzanne Benabdallah |
E-mail(s) |
nsbenabdallah@gmail.com
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Organization name |
German Cancer Research Center, DKFZ
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Department |
Functional and Structural Genomics
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Lab |
Soft-Tissue Sarcoma
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Street address |
Im Neuenheimer Feld 581 (TP4)
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City |
Heidelberg |
ZIP/Postal code |
69120 |
Country |
Germany |
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Platforms (1) |
GPL22635 |
Agilent-076323 En2_2 Custom Tiling Array G4822A (Feature number version) |
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Samples (4)
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Relations |
BioProject |
PRJNA352421 |