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Series GSE89495 Query DataSets for GSE89495
Status Public on Mar 09, 2018
Title Efficient Mammary Gland Involution Requires c-Jun N-terminal kinase
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Involution returns the lactating mammary gland to a quiescent state after weaning. The mechanism of involution involves collapse of the mammary epithelial cell compartment. To test whether the cJUN NH2-terminal kinase (JNK) signal transduction pathway contributes to involution, we established mice with JNK deficiency in the mammary epithelium. We found that JNK is required for efficient involution. JNK deficiency did not alter the STAT3/5 or SMAD2/3 signaling pathways that have been previously implicated in this process. Nevertheless, JNK promotes the expression of genes that drive involution, including matrix metalloproteases, cathepsins, and BH3-only proteins. These data demonstrate that JNK has a key role in mammary gland involution post lactation.
Overall design WAP-Cre and Jnk1f/f Jnk2f/f WAP-Cre mice were bred for a single pregnancy and litters were normalized to 6-8 pups. The pups were allowed to nurse for 9 days before forced weaning. At that point, some mice were euthanized and their mammary glands were harvested to isolate RNA (0 days). Other mice were kept for 3 days before euthanasia and mammary gland harvest (3d). In this way, gene expression differences could be determined between JNK-null and JNK-wildtype mammary glands before and during involution.
Contributor(s) Nomeda G, Roger J D
Citation(s) 29511338
Submission date Nov 03, 2016
Last update date May 15, 2019
Contact name Dr Yvonne Edwards, PhD
Organization name University of Massachusetts Medical School
Department Program in Molecular Medicine
Lab Bioinformatics Core
Street address 373 Plantation Street
City Worcester
State/province MA
ZIP/Postal code 01605
Country USA
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (12)
GSM2373896 WT_0D_1
GSM2373897 WT_0D_2
GSM2373898 WT_0D_3
BioProject PRJNA352383
SRA SRP092537

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Supplementary file Size Download File type/resource
GSE89495_BIOIFX-078_GEO_gene_expression_FINAL.xlsx 8.0 Mb (ftp)(http) XLSX
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