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Series GSE89417 Query DataSets for GSE89417
Status Public on Jan 01, 2018
Title ChIP-seq analysis of EZH1, H3K4me3 and H3K27me3 in 5F cells
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Blood develops in distinct stages. Haematopoietic progenitors in the embryo manifest restricted differentiation potential relative to definitive haematopoietic stem cells in adult bone marrow, which support lifelong multilineage haematopoiesis. To identify regulators of embryonic haematopoiesis, we screened chromatin modifiers and identified the Polycomb group protein EZH1 as a barrier to multilineage potential from pluripotent stem cells (PSCs). EZH1 was directly bound to bivalently poised, yet restricted, HSC and lymphoid genes in primitive progenitors; knockdown enabled robust generation of multilineage progenitors. Moreover, EZH1 haploinsufficiency promoted the generation of HSCs with long-term, multilineage and self-renewal potential from sites of embryonic haematopoiesis in vivo. Together, this work identifies EZH1 as a key epigenetic barrier to definitive haematopoiesis during embryonic development, and highlights the utility of chromatin modifiers as cell engineering targets to enhance blood differentiation from PSCs.
Overall design ChIP-seq was performed to determine the chromatin occupancy of FLAG-biotin-tagged EZH1, H3K4me3 and H3K27me3 in 5F cells in the presence or absence of EZH1 knockdown (shEZH1)..
Contributor(s) Vo L, Kinney M, Liu X, Zhang Y, Xu J, Daley G
Citation(s) 29342143
Submission date Nov 02, 2016
Last update date May 15, 2019
Contact name Melissa Kinney
Organization name Boston Children's Hospital
Street address 1 Blackfan Circle
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (15)
GSM2371318 ChIP-seq_5F_BirA-Only_Input
GSM2371319 ChIP-seq_5F_BirA-Only_rep1
GSM2371320 ChIP-seq_5F_BirA-Only_rep2
This SubSeries is part of SuperSeries:
GSE89418 EZH1 as a key epigenetic barrier to definitive haematopoiesis during embryonic development
BioProject PRJNA352136
SRA SRP092431

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Supplementary file Size Download File type/resource
GSE89417_RAW.tar 3.1 Gb (http)(custom) TAR (of BED, WIG)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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