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Series GSE89416 Query DataSets for GSE89416
Status Public on Jan 01, 2018
Title ATAC-seq analysis in 5F cells and AGM cells with EZH1 depletion
Organisms Homo sapiens; Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Blood develops in distinct stages. Haematopoietic progenitors in the embryo manifest restricted differentiation potential relative to definitive haematopoietic stem cells in adult bone marrow, which support lifelong multilineage haematopoiesis. To identify regulators of embryonic haematopoiesis, we screened chromatin modifiers and identified the Polycomb group protein EZH1 as a barrier to multilineage potential from pluripotent stem cells (PSCs). EZH1 was directly bound to bivalently poised, yet restricted, HSC and lymphoid genes in primitive progenitors; knockdown enabled robust generation of multilineage progenitors. Moreover, EZH1 haploinsufficiency promoted the generation of HSCs with long-term, multilineage and self-renewal potential from sites of embryonic haematopoiesis in vivo. Together, this work identifies EZH1 as a key epigenetic barrier to definitive haematopoiesis during embryonic development, and highlights the utility of chromatin modifiers as cell engineering targets to enhance blood differentiation from PSCs.
Overall design ATAC-seq was performed to determine the chromatin accessibility in 5F cells transduced with shRNAs for luciferase (control) or EZH1, or in Ezh1 WT, HET or KO embryonic day (E)10.5 AGM cells.
Contributor(s) Vo L, Kinney M, Liu X, Zhang Y, Xu J, Daley G
Citation(s) 29342143
Submission date Nov 02, 2016
Last update date May 15, 2019
Contact name Melissa Kinney
Organization name Boston Children's Hospital
Street address 1 Blackfan Circle
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
Platforms (2)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (7)
GSM2371311 ATAC-seq_5F_C11-shLuc-rep1
GSM2371312 ATAC-seq_5F_F8-shLuc-rep2
GSM2371313 ATAC-seq_5F_D4-shEZH1-rep1
This SubSeries is part of SuperSeries:
GSE89418 EZH1 as a key epigenetic barrier to definitive haematopoiesis during embryonic development
BioProject PRJNA352134
SRA SRP092430

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Supplementary file Size Download File type/resource
GSE89416_RAW.tar 1.8 Gb (http)(custom) TAR (of BED, WIG)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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