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Series GSE89371 Query DataSets for GSE89371
Status Public on Jul 17, 2017
Title A genome-wide CRISPR activation screen identifies B4GALNT2 as an inhibitory host factor for influenza virus infection
Organisms Homo sapiens; Mus musculus
Experiment type Other
Summary Identification of factors to inhibit influenza virus infection
Overall design CRISPR activation technology was used to identify genes that rendered the cell refractory to infection. The complete sgRNA library (plasmid sample) were packaged into lentivirus and used to transduce A549 cells (transduced sample). In three independent biological replicates, the transduced cells were infected with influenza virus, and the cells that were refractory to infection were collected and the sgRNAs enriched in those populations were sequenced (samples A, B, and C).
Contributor(s) Heaton NS
Citation(s) 28813663
Submission date Nov 01, 2016
Last update date May 15, 2019
Contact name Nicholas Heaton
Organization name Duke University School of Medicine
Department Molecular Genetics and Microbiology
Street address 213 Research Drive, 426 CARL Building
City Durham
State/province NC
ZIP/Postal code 27710
Country USA
Platforms (3)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
GPL16417 Illumina MiSeq (Mus musculus)
Samples (9)
GSM2367503 Sequencing of the plasmid sgRNA library_lane 1
GSM2367504 Sequencing of the plasmid sgRNA library_lane 2
GSM2367505 Sequencing of the transduced sgRNAs pre-selection_lane 1
BioProject PRJNA351924
SRA SRP092380

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE89371_Plasmid.txt.gz 930.3 Kb (ftp)(http) TXT
GSE89371_ScreenA.txt.gz 238.8 Kb (ftp)(http) TXT
GSE89371_ScreenB.txt.gz 41.4 Kb (ftp)(http) TXT
GSE89371_ScreenC.txt.gz 30.0 Kb (ftp)(http) TXT
GSE89371_TransducedLibrary.txt.gz 570.7 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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