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Series GSE8928 Query DataSets for GSE8928
Status Public on Jul 31, 2008
Title Regulation of mRNA expression of monocytic cells by stimulation with pro- abd anti-inflammatory eicosanoids
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Eicosanoids are potent regulators of gene expression of inflammatory cells. Pro- (leukotrienes B4 and C4) and anti-indflammatory (lipoxins A4 and B4) eicosanoids have been described in the literature but the detailed impact of these lipid mediators on the gene expression pattern of monocytic cells has not been studied in detail. We cultured the permanent monocytic cell line MonoMac 6 for 12 h in the absence (solvent control) and presence of these eicosanoids and quantified the differential gene expression patterns using the microarray technology.
Keywords: comparative expression studies after eicosanoid stimulation
 
Overall design MonoMac6 cells were grown according to the recommendations of the German Tissue and Cell Culture Collection (Braunschweig) to near confluency. Then 100 nM of the eicosanoids (leukotriene B4, leukotriene C4, lipoxin A4, lipoxin B4) were added to the culture medium as DMSO stock solution and the cells were further cultured for 12 h with the stimuli. Then the cells were harvested, washed and total RNA was extracted according to the Qiagen protocol. Total RNA was used for microarray hybridization.
 
Contributor(s) Heydeck D, Kuban RJ, Ungetuem U, Kuhn H
Citation(s) 15869882
Submission date Aug 31, 2007
Last update date Mar 25, 2019
Contact name Hartmut Kühn
E-mail(s) hartmut.kuehn@charite.de
Phone +49-30-450528040
Fax +49-30-450528905
Organization name Charité
Street address Monbijoustr. 2
City Berlin
State/province Berlin
ZIP/Postal code D 10117
Country Germany
 
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (5)
GSM226334 MonoMac6 - unstimulated
GSM226335 MonoMac6 - leukotriene B4
GSM226336 MonoMac6 - leukotriene C4
Relations
BioProject PRJNA102341

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Supplementary file Size Download File type/resource
GSE8928_RAW.tar 23.7 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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