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Series GSE89264 Query DataSets for GSE89264
Status Public on Jan 18, 2018
Title Transcriptional profile of mouse nephron progenitor cells
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary In developing mammalian kidney, nephron progenitor cells (NPC) give rise to all cells in mature nephrons. Expression of the transcription factor Six2 marks NPC in developing mouse kidneys. Within the Six2+ cell population, uncommitted NPC is marked by Cited1 expression. Towards the depletion of NPC in P2, most of the Cited1+ NPC is committed. Therefore, most of the Six2+ cells in kidney represents committed NPC. In order to explore the mechanism of NPC self-renewal and differentiation, hereby we generated transcriptionl profiles of uncommitted NPC (Cited1RFP+ cells from kidneys of E16.5 Cited1tagRFP transgenic mice) and committed NPC (Six2GFP+ cells from kidneys of P2 Six2TGC transgenic mice).
Overall design To obtain transcriptional profile of the target cells, we performed mRNA-Seq of cells purified from transgenic mouse kidneys (Six2TGC, Cited1tagRFP) using FACS.
Contributor(s) Guo Q, Kim A, McMahon A
Citation(s) 29449449
Submission date Oct 28, 2016
Last update date May 15, 2019
Contact name Andrew P McMahon
Phone 323-442-7847
Organization name University of Southern California
Department Stem Cell Biol & Regen Med
Lab Andrew McMahon
Street address 1425 San Pablo St, BCC 312
City Los Angeles
State/province California
ZIP/Postal code 90033
Country USA
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (11)
GSM2363335 E16.5_Six2+_mRNA_Seq_1
GSM2363336 E16.5_Six2+_mRNA_Seq_2
GSM2363337 E16.5_Six2+_mRNA_Seq_3
This SubSeries is part of SuperSeries:
GSE90017 Transcriptional and cistromic profiling of mouse nephron progenitor cells
BioProject PRJNA354556
SRA SRP093795

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Supplementary file Size Download File type/resource
GSE89264_tpm.E16Cited1+.vs.P2Six2+.all+pos+neg.xlsx.gz 2.9 Mb (ftp)(http) XLSX
GSE89264_tpm.E16Six2+.vs.E16Six2-.all+pos+neg.xlsx.gz 3.1 Mb (ftp)(http) XLSX
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Processed data are available on Series record
Raw data are available in SRA

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