NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE89125 Query DataSets for GSE89125
Status Public on Oct 24, 2016
Title Preferential Epigenetic Programming of Estrogen Response after in utero xenoestrogen (bisphenol-A) exposure [NimbleGen]
Organism Mus musculus
Experiment type Methylation profiling by genome tiling array
Summary Bisphenol-A (BPA) is an environmentally ubiquitous estrogen-like endocrine-disrupting compound. Exposure toBPAin utero hasbeen linked tofemale reproductive disorders, including endometrial hyperplasiaandbreast cancer. Estrogens are an etiological factor in many of these conditions. We sought to determine whether in utero exposure to BPA altered the globalCpGmethylation pattern of the uterine genome, subsequent gene expression, and estrogen response.Pregnantmicewere exposed to an environmentally relevant dose of BPAorDMSOcontrol.Uterine DNAand RNAwere examined by usingmethylatedDNAimmunoprecipitationmethylation microarray, expression microarray, and quantitative PCR. In utero BPA exposure altered the global CpG methylation profile of the uterine genome and subsequent gene expression. The effect on gene expression was not apparent until sexual maturation, which suggested that estrogen response was the primary alteration. Indeed, prenatal BPA exposure preferentially altered adult estrogen-responsive gene expression. Changes in estrogen response were accompanied by altered methylation that preferentially affected estrogen receptor-a (ERa)–binding genes. The majority of genes that demonstrated both altered expression and ERa binding had decreased methylation. BPA selectively altered the normal developmental programming of estrogen-responsive genes via modification of the genes that bind ERa. Gene– environment interactions driven by early life xenoestrogen exposure likely contributes to increased risk of estrogenrelateddisease in adults.—Jorgensen, E. M.,Alderman,M.H., III,Taylor, H. S. Preferential epigenetic programmingof estrogen response after in utero xenoestrogen (bisphenol-A) exposure.
 
Overall design Dams were exposed to 5 mg/kg/day BPA or DMSO control for day 9-21 of pregnancy. Offspring were euthenized at 2 weeks and their uteri harvested. Samples are pooled by litter.
 
Contributor(s) Jorgensen EM, Alderman III MH, Taylor HS
Citation(s) 27312807
Submission date Oct 24, 2016
Last update date Oct 24, 2016
Contact name Myles H Alderman III
Organization name Yale University School of Medicine
Department Cell Biology
Street address 10 Amistad St
City New Haven
State/province Ct
ZIP/Postal code 06519
Country USA
 
Platforms (1)
GPL18163 NimbleGen Mouse DNA Methylation 3x720K CpG Island Plus RefSeq Promoter Array [090618_MM9_CpG_Refseq_Prom_MeDIP]
Samples (4)
GSM2359172 CTL1
GSM2359173 CTL2
GSM2359174 BPA1
This SubSeries is part of SuperSeries:
GSE89131 Preferential Epigenetic Programming of Estrogen Response after in utero xenoestrogen (bisphenol-A) exposure
Relations
BioProject PRJNA350332

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE89125_RAW.tar 197.8 Mb (http)(custom) TAR (of GFF, PAIR)
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap