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Series GSE89061 Query DataSets for GSE89061
Status Public on Jul 01, 2018
Title In utero exposure to therapeutic doses of acetaminophen and ibuprofen accelerates germ cell differentiation in the mouse embryonic testis
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary NSAIDs and ACE that affect prostaglandin synthesis are widely used by pregnant women. Epidemiological studies have hypothesized a potential relation of testis dysgenesis syndromes such as cryptorchidism and hypospadias to exposure to these molecules during both the first and the second trimesters of gestation. To decipher whether the embryonic gonads themselves are targets for these molecules, we analysed the impact of precocious in utero exposure to NSAIDs and ACE alone or in combination on the early development of the testis during sex determination, using therapeutic doses similar to those administrated in human medications. We found that in utero exposure to ACE, aspirin or ibuprofen affects the germ cell proliferation in embryonic testis. The whole transcriptome of 13.5 dpc (days post coïtum) treated testis suggests different mechanisms of action of these drugs and a functional interaction between both molecules used in combination, in accelerating the germ cell differentiation. We identified that ACE and ibuprofen exposure through the up-regulation of Dnmt3L expression induces advanced epigenetic reprograming of the germline and enhanced glycogen storage within the testis cords through the activation of extracellular matrix genes expression. In addition, we identified for the first time the prostaglandin production pattern in the embryonic gonad and showed that PGD2, PGE2 and PGI2 were the targets of ACE and NSAIDs drugs. These features might affect the formation and maturation of postnatal testis and secondary reproductive organs leading to male infertility in adult age.
Overall design examination of the impact of in utero exposure to NSAIDs and ACE on testis organogenesis
Contributor(s) Rossito M, Pruvost A, Marchive C, Ghettas A, Poulat F, Philibert P, Boizet-Bonhoure B
Citation(s) 29979629
Submission date Oct 21, 2016
Last update date May 15, 2019
Contact name Brigitte Boizet-Bonhoure
Phone 33 4 34 35 99 40
Organization name CNRS
Department Genetic and Development
Street address 141 rue de la Cardonille
City Montpellier
ZIP/Postal code 34396
Country France
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (4)
GSM2358099 Control
GSM2358100 ACE+ACE
GSM2358101 ASP+ACE
BioProject PRJNA350220
SRA SRP091955

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE89061_ACE+ACE_vs_ACE+IBU.txt.gz 8.5 Kb (ftp)(http) TXT
GSE89061_ACE+ACE_vs_ASP+ACE.txt.gz 1.1 Kb (ftp)(http) TXT
GSE89061_ASP+ACE_vs_IBU+ACE.txt.gz 6.5 Kb (ftp)(http) TXT
GSE89061_Control_vs_ACE+ACE.txt.gz 2.6 Kb (ftp)(http) TXT
GSE89061_Control_vs_ASP+ACE.txt.gz 1.7 Kb (ftp)(http) TXT
GSE89061_Control_vs_IBU+ACE.txt.gz 6.8 Kb (ftp)(http) TXT
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Processed data are available on Series record

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