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Series GSE89032 Query DataSets for GSE89032
Status Public on Oct 20, 2017
Title Post-transcriptional Gene Silencing Mediated by microRNAs is Controlled by Nucleoplasmic Sfpq [RNA-Seq]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary There is a growing body of evidence about the presence and the activity of the miRISC in the nucleus of mammalian cells. Here, we show by quantitative proteomic analysis that Ago2 interacts with nucleoplasmic Sfpq in a RNA-dependent fashion. By HITS-CLIP and transcriptomic analyses, we demonstrated that Sfpq directly controls the miRNA targeting of a subset of crucial miRNA-target mRNAs when it binds locally. Sfpq modulates miRNA targeting in both nucleoplasm and cytoplasm, indicating a nucleoplasmic imprinting of Sfpq-target mRNAs that influence miRNA targeting in both cellular compartments. Mechanistically, Sfpq binds to a sizeable set of long 3’UTR forming long aggregates to optimize miRNA position/recruitment to selected binding sites, as we show for Lin28A mRNA. These results extend the miRNA-mediated post-transcriptional gene silencing into the nucleoplasm and indicate that an unique Sfpq-dependent post-transcriptional strategy for controlling both nuclear and cytoplasmic gene expression takes place in cells during physio-pathological events.
Overall design RNA-seq of P19 cells control and upon SFPQ knockdown both in triplicates
Contributor(s) Bottini S, Hamouda-Tekaya N, Mategot R, Zaragosi L, Audebert S, Grandjean V, Mauduit C, Benahmed M, Barbry P, Repetto E, Trabucchi M
Citation(s) 29084942
Submission date Oct 21, 2016
Last update date May 15, 2019
Contact name Silvia Bottini
Organization name C3m
Street address 299, chemin de la ginestiere
City Nice
ZIP/Postal code 06200
Country France
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (6)
GSM2357690 CTRL_sample1
GSM2357691 CTRL_sample2
GSM2357692 CTRL_sample3
This SubSeries is part of SuperSeries:
GSE89033 Post-transcriptional Gene Silencing Mediated by microRNAs is Controlled by Nucleoplasmic Sfpq
BioProject PRJNA349978
SRA SRP091919

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Supplementary file Size Download File type/resource
GSE89032_differential_spliced_events.xlsx.gz 31.1 Kb (ftp)(http) XLSX
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Raw data are available in SRA

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