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Series GSE89011 Query DataSets for GSE89011
Status Public on Jan 06, 2017
Title Nat1 promotes translation of specific proteins that induce differentiation of mouse embryonic stem cells [ribosome profiling]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Nat1 (also known as p97/Dap5/Eif4g2) is a ubiquitously expressed cytoplasmic protein that is homologous to the C-terminal two thirds of eukaryotic translation initiation factor 4G (also known as Eif4g1). We previously showed that Nat1-null mouse embryonic stem cells (mESCs) were resistant to differentiation. In the current study, we found that Nat1 and Eif4g1 share many binding proteins, such as Eif3s, Eif4s and ribosomal proteins. However, Nat1 did not bind to Eif4e or poly A binding proteins, which are critical for cap-dependent translation initiation. In contrast, Nat1 binds to Eif2s, Fmr and related proteins, and Prrc2 proteins more preferentially than does Eif4g1. We also found that Nat1-null mESCs possess a status partially similar to ground state, which is established in wild-type mES cells when treated with inhibitors of the ERK and GSK3 signaling pathways. In Nat1-null mESCs, the ERK pathway is suppressed even without inhibitors. Ribosomal profiling revealed that translation of Map3k3 and Sos1 is suppressed in the absence of Nat1. Forced expression of Map3k3 induced differentiation of Nat1-null mESCs. These data collectively showed that Nat1 is involved in translation of proteins that are required for cell differentiation.
Overall design Examining ribosome-protected mRNA fragments (RPF) and total RNA in replicate WT ESCs (n=2), Nat1+/- mESCs (n=3) and Nat1-null ESCs (n=3).
Contributor(s) Sugiyama H, Takahashi K
Citation(s) 28003464
Submission date Oct 20, 2016
Last update date May 15, 2019
Contact name Shinya Yamanaka
Phone 81-75-366-7041
Organization name Center for iPS Cell Research and Applicaton (CiRA), Kyoto University
Street address 53 Kawahara-machi, Shogoin, Sakyo-ku
City Kyoto
ZIP/Postal code 606-8507
Country Japan
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (16)
GSM2357212 WT RPF rep1
GSM2357213 WT Total rep1
GSM2357214 WT RPF rep2
This SubSeries is part of SuperSeries:
GSE89012 Nat1 promotes translation of specific proteins that induce differentiation of mouse embryonic stem cells
BioProject PRJNA349752
SRA SRP091889

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Supplementary file Size Download File type/resource
GSE89011_NormalizedCounts.txt.gz 1.3 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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