GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE88997 Query DataSets for GSE88997
Status Public on Mar 14, 2017
Title Reconstituting development of pancreatic intraepithelial neoplasia from primary human pancreas duct cells
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Development of systems that reconstitute hallmark features of human pancreatic intraepithelial neoplasia (PanINs), the precursor to pancreatic ductal adenocarcinoma, could generate new strategies for early diagnosis and intervention. However, human cell-based PanIN models with defined mutations are unavailable. Here, we report that genetic modification of primary human pancreatic cells leads to development of lesions resembling native human PanINs. Primary human pancreas duct cells harbouring oncogenic KRAS and induced mutations in CDKN2A, SMAD4 and TP53 expand in vitro as epithelial spheres. After pancreatic transplantation, mutant clones form lesions histologically similar to native PanINs, including prominent stromal responses. Gene expression profiling reveals molecular similarities of mutant clones with native PanINs, and identifies potential PanIN biomarker candidates including Neuromedin U, a circulating peptide hormone. Prospective reconstitution of human PanIN development from primary cells provides experimental opportunities to investigate pancreas cancer development, progression and early-stage detection.
Overall design We performed RNAseq analysis using KCT, KCST, KECST clones along with donor-matched control ductal sphere cells (S1-CTRL, S1 KCSTclone3, S1 KECSTclone3, S1 KCSTclone4, S1 KECSTclone4, S2 KCTclone3, S2 KCSTclone8, S2 KECSTclone8).
Contributor(s) Lee J, Kim SK
Citation(s) 28272465
Submission date Oct 20, 2016
Last update date May 15, 2019
Contact name Jonghyeob Lee
Organization name Stanford University School of Medicine
Street address 279 Campus Drive, Beckman Center B300
City Stanford
State/province CA
ZIP/Postal code 94305
Country USA
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (8)
GSM2356897 S2-KCTclone3
GSM2356898 S2-KCSTclone8
GSM2356899 S2-KECSTclone8
BioProject PRJNA349475
SRA SRP091873

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE88997_Lee-Total-Raw-Count.txt.gz 696.9 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap