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| Status |
Public on Feb 05, 2016 |
| Title |
Flagellin-mediated effects of Salmonella enteritidis on gene expression in intestinal epithelial cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Analysis of gene expression in Caco-2 intestinal epithelial cells stimulated with flagellated or aflagellated S. enteritidis or recombinant flagellin. Results provide insight into how flagellin mediates innate immune responses in intestinal epithelial cells.
Keywords: Gene expression analysis
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| Overall design |
Caco-2 cells were grown in 100 mm dishes to 3 days post-confluence. Cells were then incubated for 2 h with the following four treatments: medium alone (control); flagellated S. enteritidis; aflagellated S. enteritidis; or recombinant flagellin. Three independent experiments were performed to give an N=3 for each treatment. Caco-2 RNA was extracted and hybridized to the Affymetrix GeneChip Human Genome U133 Plus 2.0 Array.
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| Contributor(s) |
Kelly D, Mulder IE |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Aug 29, 2007 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Denise Kelly |
| E-mail(s) |
D.Kelly@rri.sari.ac.uk
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| Phone |
+44 1224 716648
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| Organization name |
University of Aberdeen
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| Lab |
Gut Immunology Group
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| Street address |
Institute of Medical Sciences
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| City |
Aberdeen |
| ZIP/Postal code |
AB25 2ZD |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA102285 |
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