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Series GSE88953 Query DataSets for GSE88953
Status Public on Feb 21, 2018
Title Impact of hypothermic preservation of mouse kidney resident immune cells
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary With the growing interest in studying primary tissue samples by single cell transcriptome analysis, there is an emerging demand for a preservation strategy that enables sample transportation and storage. In this study, we describe a simple and general strategy that preserves primary tissues at hypothermic temperature. Using FACS and single-cell RNAseq, we demonstrated the effectiveness of this strategy in maintaining cell viability, cell population heterogeneity, and cell transcriptome integrity for primary tissues that underwent up to 3 days of preservation.
Overall design Examine the impact of hypothermic preservation on mouse kidney resident immune cells over up to 4 days at single-cell resolution
Contributor(s) Wang W, Quake SR
Citation(s) 29439658
Submission date Oct 19, 2016
Last update date May 15, 2019
Contact name Wanxin Wang
Phone 4134373134
Organization name Stanford Univeristy
Lab Wanxin Wang
Street address James H Clark Center, 318 Campus Drive, room E300
City Stanford
State/province CA
ZIP/Postal code 94305
Country USA
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (502)
GSM2355788 6hour_A10__IM793-727-502_S106
GSM2355789 6hour_A11__IM793-728-502_S107
GSM2355790 6hour_A12__IM793-729-502_S108
BioProject PRJNA349178
SRA SRP091780

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Supplementary file Size Download File type/resource
GSE88953_count_502.csv.gz 2.7 Mb (ftp)(http) CSV
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