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Series GSE88914 Query DataSets for GSE88914
Status Public on Feb 22, 2017
Title Evaluation of three small RNA library preparation kits on serum-derived RNA
Organism Mus musculus
Experiment type Non-coding RNA profiling by high throughput sequencing
Summary We evaluated the effect of the small RNA library preparation method on 5' tRNA-halves and miRNA abundance in libraries prepared from serum RNA using three commercially available small RNA library preparation kits (TruSeq small RNA library preparation kit v2 (Illumina), TailorMix miRNA sample preparation kit v2 (Seqmatic) and the NEBNext Multiplex Small RNA library prep kit (New England Biolabs)). RNA isolated from 100 ┬Ál of serum collected from healthy mice was used as input for the preparation of a small RNA library in duplicate and libraries were single end sequenced.
 
Overall design Serum samples from healthy mice were used to evaluate three different small RNA library preparation kits. Each kit was evaluated in duplicate.
 
Contributor(s) Van Goethem A
Citation(s) 27901112
Submission date Oct 18, 2016
Last update date May 15, 2019
Contact name Alan Van Goethem
Organization name Ghent University
Street address De Pintelaan 185
City Ghent
ZIP/Postal code 9000
Country Belgium
 
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (6)
GSM2354265 Serum_1. Truseq
GSM2354266 Serum_2. Truseq
GSM2354267 Serum_1. TailorMix
This SubSeries is part of SuperSeries:
GSE88915 Depletion of tRNA-halves enables effective small RNA sequencing of low-input murine serum samples
Relations
BioProject PRJNA349074
SRA SRP091730

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE88914_RAW.tar 740.0 Kb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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