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Series GSE88879 Query DataSets for GSE88879
Status Public on Oct 19, 2016
Title Long Term Culture of the A549 Cell Line Promotes Differentiation Towards an Alveolar Type II (ATII) Phenotype; A549 Time course.
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Pulmonary research requires models that represent the physiology of alveolar epithelium but concerns with reproducibility, consistency and the technical and ethical challenges of using primary or stem cells has resulted in widespread use of continuous cancer or other immortalized cell lines. The A549 ‘alveolar’ cell line has been available for over four decades but there is an inconsistent view as to its suitability as an appropriate model for primary alveolar type II (ATII) cells. Since most work with A549 cells involves short term culture of proliferating cells, we postulated that culture conditions that reduced proliferation of the cancer cells would promote a more differentiated ATII cell phenotype. We examined A549 cell growth in different media over long term culture and then used microarray analysis to investigate temporal regulation of pathways involved in cell cycle and ATII differentiation; we also made comparisons with gene expression in freshly isolated human ATII cells. Analyses indicated that long term culture in Ham’s F12 resulted in substantial modulation of cell cycle genes to result in a quiescent population of cells with significant up-regulation of autophagic, differentiation and lipidogenic pathways. There were also increased numbers of up- and down-regulated genes shared with primary cells suggesting adoption of ATII characteristics and multilamellar body (MLB) development. Subsequent Oil Red-O staining and Transmission Electron Microscopy confirmed MLB expression in the differentiated A549 cells. This work defines a set of conditions for promoting ATII differentiation characteristics in A549 cells that may be advantageous for studies with this cell line.
Overall design The A549 Cell Line was maintained in long term culture in Ham's F12 medium with 10% Foetal Bovine Serum for 25 days in triplicate in order to drive differentation towards an alveolar type II differentated state. Cells were harvested at T0 (log phase), day 7, 11, 18 and 25 to evaluate their gene expression over the differentation period.
Contributor(s) Cooper JR
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Submission date Oct 18, 2016
Last update date Jan 09, 2018
Contact name James Ross Cooper
Phone +44 1980 612707
Organization name Public Health England
Department ECACC
Lab Scientific Development Group
Street address PHE Porton, Manor Farm Road
City Salisbury
State/province Wiltshire
ZIP/Postal code SP4 0JG
Country United Kingdom
Platforms (1)
GPL17077 Agilent-039494 SurePrint G3 Human GE v2 8x60K Microarray 039381 (Probe Name version)
Samples (15)
GSM2350653 Log Phase A549 Cells Replicate 1
GSM2350654 Log Phase A549 Cells Replicate 2
GSM2350655 Log Phase A549 Cells Replicate 3
This SubSeries is part of SuperSeries:
GSE88881 Long Term Culture of the A549 Cell Line Promotes Differentiation Towards an Alveolar Type II (ATII) Phenotype
BioProject PRJNA349020

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE88879_RAW.tar 45.3 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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