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Series GSE88858 Query DataSets for GSE88858
Status Public on Nov 15, 2016
Title RNA-Seq gene profiling comparison from human cDC subsets and pre-cDC subsets
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary The goal of this study is to compare the gene profiling of human blood BDCA-1+ cDCs, blood BDCA-3+ cDCs, CB CD172a-pre-cDCs and CB CB172a+ pre-cDCs. RNA from sorted populations was extracted and column-purified using the Arcturus PicoPure RNA Isolation kit (Applied Biosystems). Genomic DNA was removed by on-column digest with DNAse I (Qiagen), according to the PicoPure RNA Isolation kit manual. RNA libraries were prepared using the SMARTer Ultra Low Input RNA for Sequencing kit (Clontech Laboratories) followed with Nextera XT DNA Library Prep kit (Illumina). Libraries were sequenced by 75-bp single-end reading on a NextSeq 500 sequencer (Illumina). Our results show that CD172a+ and CD172a- pre-cDCs represent developmentally discrete populations that differentially express lineage-restricted transcription factors. Moreover CD172a- pre-cDCs cluster with BDCA-3hi cDCs and CD172a+ pre-cDCs with BDCA-1+ cDCs.
Overall design RNA from sorted cord blood pre-cDC subsets (150-400 cells) and peripheral blood cDC subsets (2000-10000 cells). Done in quadruplicate.
Contributor(s) Breton G, Tojal da Silva I, Valieris R
Citation(s) 27864467
Submission date Oct 18, 2016
Last update date May 15, 2019
Contact name Israel Tojal da Silva
Phone 1121895000
Organization name HOSPITAL A C CAMARGO
Street address Tagua, 440
City Sao Paulo
State/province SP
ZIP/Postal code 01508-010
Country Brazil
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (16)
GSM2350346 Blood BDCA-1+DCs Rep1
GSM2350347 Blood BDCA-1+DCs Rep2
GSM2350348 Blood BDCA-1+DCs Rep3
BioProject PRJNA348814
SRA SRP091695

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Supplementary file Size Download File type/resource
GSE88858_RAW.tar 1.5 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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