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Series GSE88802 Query DataSets for GSE88802
Status Public on Oct 18, 2016
Title Novel metastatic models of human esophageal adenocarcinoma highlight the importance of E-cadherin in cancer metastasis
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary We report the identification of FLO-1, and derivation of its isogenic companion FLO-1LM, as two spontaneously metastatic cell line models of human esophageal adenocarcinoma (EAC). We performed genome-wide RNAseq comparing FLO-1LM and FLO-1 parental cells to gain molecular insights into the phenotypic differences between these two cell lines. Using an adjusted p-value<0.05, a total of 375 (307 downregulated, 68 upregulated) genes were differentially regulated in FLO-1LM compared with parental cells. Genome-wide RNAseq profiling revealed significant enrichment of metastasis-related pathways in FLO-1LM cells, many of which are also evident in patient samples. Moreover, CDH1, which encodes the adhesion molecule E-cadherin, was the most significantly downregulated gene in FLO-1LM compared to FLO-1.
Overall design Genome-wide RNAseq comparing FLO-1LM and FLO-1 parental cells
Contributor(s) Liu DS, Hoefnagel SJ, Fisher OM, Krishnadath KK, Montgomery KG, Busuttil R, Colebatch A, Read M, Duong CP, Philips WA, Clemons NJ
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Submission date Oct 17, 2016
Last update date May 15, 2019
Contact name Sanne Hoefnagel
Organization name Academic Medical Centre Amsterdam
Department Gastroenterology
Lab CEMM Krishnadath
Street address Meibergdreef 9
City Amsterdam
State/province Noord-Holland
ZIP/Postal code 1105AZ
Country Netherlands
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (4)
GSM2348284 102842-001-048
GSM2348285 102842-001-049
GSM2348286 102842-001-050
BioProject PRJNA348681
SRA SRP091645

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Supplementary file Size Download File type/resource
GSE88802_RAW.tar 850.0 Kb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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