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Series GSE87877 Query DataSets for GSE87877
Status Public on Oct 15, 2016
Title Cell type-specific chromatin states differentially prime squamous cell carcinoma tumor-initiating cells for epithelial to mesenchymal transition [expression 1]
Organism Mus musculus
Experiment type Expression profiling by array
Summary Epithelial to mesenchymal transition (EMT) in cancer cells has been associated with metastasis, stemness and resistance to therapy. The reason why some tumors undergo EMT and other not might reflect intrinsic properties of their cell of origin, although this possibility is largely unexplored. By targeting the same oncogenic mutations to discrete skin compartments, we show cell type-specific chromatin and transcriptional states differentially prime tumors to EMT. Squamous cell carcinomas (SCCs) derived from intrafollicular epidermis (IFE) are generally well-differentiated, while hair follicle (HF) stem cell-derived SCCs frequently exhibit EMT, efficiently form secondary tumors, and possess increased metastatic potential. Transcriptional and epigenomic profiling revealed IFE and HF tumor-initiating cells possess distinct chromatin landscapes and gene regulatory networks associated with tumorigenesis and EMT that correlate with accessibility of key epithelial and EMT transcription factor binding sites. These findings highlight the importance of chromatin states and transcriptional priming in dictating tumor phenotypes and EMT.

Overall design KrasG12D and YFP expression combined with deletion of p53 were induced in Hair Follicle Stem cells (SCs) and their progeny using Lgr5CREER/KRasG12D/p53fl/fl/Rosa-YFP mice, and in the Intra Follicular Epidermis and the infundibulum using K14CREER/KRasG12D/p53fl/fl/Rosa-YFP mice by intraperitoneal tamoxifen injection. Cells were harvested and compared prior oncogenic hits and in fully grown tumours.
Contributor(s) Latil ML, Nassar D, Beck B, Boumhadi S, Brisebarre A, Blanpain C
Citation(s) 27889319
Submission date Oct 12, 2016
Last update date Feb 11, 2019
Contact name C├ędric Blanpain
Organization name ULB
Street address Route de Lennik 808
City Brussels
ZIP/Postal code 1070
Country Belgium
Platforms (2)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
GPL11180 [HT_MG-430_PM] Affymetrix HT MG-430 PM Array Plate
Samples (16)
GSM1841774 307 2014 K14 Ep+
GSM1841775 308 2014 K14 Ep+
GSM1841778 605 2015 Lgr5 Ep+ MIXED
This SubSeries is part of SuperSeries:
GSE71621 Cell type-specific chromatin states differentially prime squamous cell carcinoma tumor-initiating cells for epithelial to mesenchymal transition
BioProject PRJNA348518

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE87877_RAW.tar 37.6 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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